Viro 2/E1

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TCID50-

50% Tissue Culture Infectious Dose
ID50-

50% Infectious Dose
LD50-

Lethal Dose
MOI-
- Multiplicity of Infection
- Number of Virus Particle provided for Each Cell
Virus infection of cultured cells appears as…
- General cell death
- Plaque formation
- Syncytial formation
- Formation of inclusion bodies
- Cell transformation
- Persistent infection
Cell damage by viruses
- Accumulation of viral structural components
- Formation of virion aggregates within the cell
- Shutdown of cellular protein synthesis
Isolation is utilized for…

Immunological Detection
Quatitation is utilized for…
- TCID50
- Plaque Assay
Which Test is frequently used in the lab and in clinics

ELISA
Types of Virus Titration:
- Plaque
- Pock
- Transformation
- Hemaglutination/HI
What tests are used to determine TCID50?
- Plaque
- Pock
- Transformation
- (Titration)
Bovine Herpes Virus can be detected using…
- ELISA
- Immunofluorescence
- Immunohistochemistry
What Viruses are often tested in clinics, using ELISA(8)?
- FeLV
- FIV
- Parvovirus
- Bovine Herpes virus
- Bovine viral diarrhea virus
- Equine Infectious Anemia virus
- Influenza-A
- Porcine Respiratory Reproductive Syndrome Virus
What Tests Can Detect Viral Nucleic Acids?
- Hybridization
- PCR
- Nucleic Sequencing
Methods for Virus Isolation
- Cell Culture
- Animal Inoculation
- Embryonated Egg Inoculation
What Methods are used to ID viral isolates?
- Virusneutralization
- Hemagglutination/Inhibition
- Hemadsorption and hemadsorption inhibition
- Complementfixation
- Immunodiffusion
- Restriction endonuclease emapping
- Radiolabeling of Protein and Immunoprecipitation
- Western Blot Analysis
+HG=

No button
+HAI=

Button
Complement Fixation Test=> Lysis of RBC’s. This indicates that…

No complement
Methods Used to Detect exposure (3)
- (Serology)
- Virus neutralization
- Hemagglutination inhibition
- ELISA
Limitations of serology
- Detects exposure not when exposure occurred
- For correlation with disease
- Paired sera
- IgM
- CSF

Author

HLW

98459

Card Set

Viro 2/E1

Description

Viro 2/E1

Updated

2011-08-27T19:15:51Z

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