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Viro 2/E1
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TCID50-
50% Tissue Culture Infectious Dose
ID50-
50% Infectious Dose
LD50-
Lethal Dose
MOI-
Multiplicity of Infection
Number of Virus Particle provided for Each Cell
Virus infection of cultured cells appears as…
General cell death
Plaque formation
Syncytial formation
Formation of inclusion bodies
Cell transformation
Persistent infection
Cell damage by viruses
Accumulation of viral structural components
Formation of virion aggregates within the cell
Shutdown of cellular protein synthesis
Isolation is utilized for…
Immunological Detection
Quatitation is utilized for…
TCID50
Plaque Assay
Which Test is frequently used in the lab and in clinics
ELISA
Types of Virus Titration:
Plaque
Pock
Transformation
Hemaglutination/HI
What tests are used to determine TCID50?
Plaque
Pock
Transformation
(Titration)
Bovine Herpes Virus can be detected using…
ELISA
Immunofluorescence
Immunohistochemistry
What Viruses are often tested in clinics, using ELISA(8)?
FeLV
FIV
Parvovirus
Bovine Herpes virus
Bovine viral diarrhea virus
Equine Infectious Anemia virus
Influenza-A
Porcine Respiratory Reproductive Syndrome Virus
What Tests Can Detect Viral Nucleic Acids?
Hybridization
PCR
Nucleic Sequencing
Methods for Virus Isolation
Cell Culture
Animal Inoculation
Embryonated Egg Inoculation
What Methods are used to ID viral isolates?
Virusneutralization
Hemagglutination/Inhibition
Hemadsorption and hemadsorption inhibition
Complementfixation
Immunodiffusion
Restriction endonuclease emapping
Radiolabeling of Protein and Immunoprecipitation
Western Blot Analysis
+HG=
No button
+HAI=
Button
Complement Fixation Test=> Lysis of RBC’s. This indicates that…
No complement
Methods Used to Detect exposure (3)
(Serology)
Virus neutralization
Hemagglutination inhibition
ELISA
Limitations of serology
Detects exposure not when exposure occurred
For correlation with disease
Paired sera
IgM
CSF
Author
HLW
ID
98459
Card Set
Viro 2/E1
Description
Viro 2/E1
Updated
2011-08-27T19:15:51Z
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