Using the pour plate technique, describe how you would do a viable plate count. Include the materials needed and the limitation necessary to obtain an acceptable bacterial count. Also, what are the consequences of exceeding the required limitations.
Use the same materials and techniques for the pour plate technique. Dilutions 1-100 to 1-1000 are made. As the agar hardens individual baterial cells are fixed and form colonies upon incubation. Surface colony counts should be between 100-200 CFU per plate. If below or to high (above 250 cfu per plate) then a statistical problem.