Micro 17 & 19

Read at the bottom of the miniscus

• Stock broth culture, saline tubes, pipette, pipetter, pretri dishes, and tubes of melted agar
• -1mL of broth culture is transfered to 1st saline tube with a pipette, specimen is mixed in the saline tube.
• -1mL of saline mix in 1st tube is transferred to 1st petri dish, go back into the 1st saline tube and transfer 1mL of saline mix to the 2nd tube of saline and mix.
• -transfer 1mL of saline mix in 2nd tube to 2nd petri dish go back into 2nd saline tube and transfer 1mL of saline mix to 3rd saline tube and mix.
• -transfer 1mL of 3rd tube mix to 3rd petri dish, pour melted agar into each petri dish
• -mix let cool to harden and incubate overnight

Use the same materials and techniques for the pour plate technique. Dilutions 1-100 to 1-1000 are made. As the agar hardens individual baterial cells are fixed and form colonies upon incubation. Surface colony counts should be between 100-200 CFU per plate. If below or to high (above 250 cfu per plate) then a statistical problem.

• -More room to grow on the sruface than in the agar.
• -toxic by products are diluted on the surface better
• -more nutrients are available on the surface as the bacteria grows

• -Bacterial cocci is the proper name for spherical shaped bacteria
• -Baccili is the proper name for rod shaped bacteria
• -sprilli/spirochae is the proper name for corkscrew shaped bacteria