Microbiology

PEA
- Gram positive selective.
- Gram negative - No growth
MSA
- Salt tolerant Staphlococcus
- S. aureus will show yellow
- Growth with no color change - Other staph
- Gram neg no growth
BAP
- Nonselective - Gram pos and neg
- Hemolysis
- Beta - Complete hemolysis
- Alpha - Partial hemolysis
- Gamma - No hemolysis
EMB
Eosin Methlyne Blue Agar
- Gram neg - Differentiates between LF (coliforms) and NLF.
- Gram Pos - No growth
- Gram Neg LF - Purple/Green sheen
- Gram Neg NLF - Pink
MAC
Maconkey
- Same as EMB
- Gram Pos - No growth
- Gram Neg LF - Dark Pink (DARK)
- Gram Neg NLF - Colorless
HE
Hektoen Enteric Agar
- Selective and Differential for
- Salmonella and Shigella
- Gram Pos - No growth
- Gram Neg - VARIES
- Salmonella and Shigella - Blue/Green
- H2s - Black colonies
Centrimide Agar
- Selective for Psudomonas auruginosa
- If Psudomonas auruginosa present growth present - Green pigment is called Pyocyanin - Illuminates under UV light
E-Coli

Gram Negative Bacteria
Staph aureus

Gram Positive
Bacillus

Gram Positive Spore Forming
Iodine is what part of Gram Stain?

Mordant
Residurin Indicator turns what color when exposed to O2?

Turns Pink

Methaline Blue turns Blue
Postively Charged Dyes
- Saffarin
- Crystal Violet
- Methaline Blue
Negatively Charged Dye(s)

Nigrosin
What is a faucaltive anarobe

Does not need oxygen to survive but preffers it.

Author

Johng9999

89643

Card Set

Microbiology

Description

Agar Plates

Updated

2011-06-09T10:34:41Z

Show Answers