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Domain Bacteria
True Bacteria
Peptidoglycan cell wall
Contains all the pathogentic prokaryotes and most soil and water bacteria.
Domain Eukarya includes what 4 kingdoms?
Protista, Fungi, Plants and Animals
Protista
Unicellular
Have flagella sometime during life cycle
Include protozoa
Fungi
Incl. unicellular yeast and multicellular fungi (mushrooms we eat)
Absorbs organic matter through plasma membrane
Cells in multicellular fungi joined to form hyphae
Most fungi lack flagella
Fungi develop from spores or other hyphae fragments
Plantae
Include multicellular algae
Use photosynthesis for energy
Animalia
Include worms
Obtain nutrients and energy by ingesting organic matter through a mouth of some kind.
Scientific Nomenclature
Binomial
Genus species
Methods of classifying and identifying Microorganisms
K
ings
P
lay
C
hess
O
n
F
ine
G
rain
S
and
Kingdom
Phylum
Class
Order
Family
Genus
Species
Classification
What scientists do
List of characteristics and means for comparison to aid in
identification - Bergey's Manual
Identification
What we do
Figuring out what organism it is for practical purposes of treatments
of infection. Not necessarily done with same means as classification.
Differential staining
One of first steps for ID
Gram stain - 1st
Acid fast stain - TB
Biochemical Tests
The possession of various enzymes as determined by biochemical tests.
Also selective and differential media is used.
Ex: peroxide test - did it bubble or not?
Serology
Use antibodies in a variety of ways to either detect presence of
microorganisms or past infections.
DNA fingerprinting, Polymerase Chain Reaction (PCR)
Can tell relatedness of organisms. Improves classification schemes.
DNA fingerprinting used in epidemiological studies of source of outbreaks.
PCR used to detect small amounts of organisms or organisms not detectable
by conventional culture techniques.
(you can get some false neg. & false pos.)
Staphylococcus
Gram-Positive Cocci
Occur in grape like clusters
Staphylococcus aureus - named for golden color
Causes many diseases such as food poisoning and toxic
shock syndrome.
Staphylococcus Characteristics
Grow under high osomotic pressure and low moisture. Grows on Mannitol salt agar
and Staph aureus ferments mannitol.
Produce toxins that contribute to pathogenicity-enterotoxin involved
Author
Anonymous
ID
8785
Card Set
microbiology
Description
Test II
Updated
2010-03-02T06:06:22Z
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