Virology Parvovirus

small because it is the smallest virion

naked icosahedral

linear ssDNA (+ or -) with self complementary hairpin structures at terminus that act as primers.

• Stronge dependancy on cell cycle
• Autonomous parvo replicates only in S-phase
• Dependo- requires a helper virus
• DNA must first become double stranded before trx
• Site specific integration (adeno-associated virus)

• (AAV)
• -Adenovirus or Herpesvirus

establishes a latent infection by inserting its genome because Rep78 binds, cleaves and promotes integration.

• E1A (transcriptional activator of AAV genes and cellular DNA replication genes)
• E1B ( transports AAV mRNA to cytoplasm for Tln)

• 1. Hair pin (ABCA' [3' end] with palandrom at other end
• ABCA')
• 2. Host DNA pol extends 3' end (has -OH)
• 3. Rep nicks at A' of 3' end so hairpin can unfold and be copied on new stand
• 4. Hair pins reform (on old and new strands)

AAV