Lab test 1

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What is the primary stain in gram staining

Crystal violet
What is the mordant in gram staining

Grams iodine
What is the decolorizer in gram staining95/ethanol

95% ethanol
What is the counter stain in gram staining

safranin
E.coli is gram neg

Red rod
Bac. Cer is gram pos

Blue rod
Staphylococcus aureus is gram pos

Blue grape like cluster of cocci
What stain is used in negative staining

Nigrosine
Negative stain bac. Cer. Looks like

Long rods
Negative stain sar. Lut. Looks

Small cluster-cellular arrangements called tetrads
What is the backround in neg staining

Black
What is the primary stain in a capsule stain

1% crystal violet
What is the wash in a capsule stain

20 % Copper sulfate
What does strep. Lactose look like in a capsule stain

Purple diplococci in white capsule, purple backround
What is the primary stain in a acid- fast stain

Carbolfuchsin
What is the mordant in acid- fast stain

Steam heat
What is the decolorizer in acid-fast stain

Acid alcohol =HCL+95%ethanol
What is the counter stain in acid-fast staining

Methylene blue
What bacterium is mycobacterium smegmatis

Fushia rods
What is the primary stain in spore staining

Malichite green
What is the mordant in spore staining

Steam heat
What is the decolorizer in spore staining

Water
What is the counterstain in spore staining

Safranin
What does bac.cer. Look like in a spore stain

Green spores on red rods / endospores
seratia marcescen in a pure culture is

Red
In a pure culture method what color is sarcina lutea

Canary yellow
Pure culture method petri plate, cac. cer looks

billowey
Thioglycollate Broth is used to

take ou O2 gas
What does Thioglycollate broth grow

anearobes and gaculitative bacteria
What gasius requirerment is clostridium sporogenes

anaerobic - tube
What gasius requirerment is escheria coli

facualtative
What gasius requirerment is Sarc. lut

aerobic
What gasius requirerment is bas sub

aerobic
What is used in the wright's tube test

cotton ball, 10% NaOH(sodium Hydroxide) and Pyrogallic acid is the acid used
Describe Psuedomonas eruginose

has a fruity odor and turns agar green due to green water soluble pigment
What bedia is used in to test mobility

motility test agar
What is the PH indicator in sugar fermentation

Phenal red
Phenal red in a neutral

red
phenal red in a base

red
phenal red in a acid

yellow
phenol red in a alkaline

yellow
What is the small tube inside the test tube called

Durham tube
What enzyme do we look for in starch hydrolysis test

Starch Hydrolase
What is the regeant used in starch hydrolysis test

iodine
What is the media used in starch hydroliysis test

starch agar
Bacillius subt. is positive in starch agar

shows a light halo around bacteia and purple background
Staph aur. is negative in starch agar

shows purple throughout the petri dish
What enzyme is tested for in Urea Hydrolysis

urease
What media is used in urea Hydrolysis test

urea broth
What is the PH indicator in urea hydroloysis

phenol red
Prot. vulg. is positive for urease when it

changes to cerise in color
What is the substrat in litmus milk

litmus milk
What is the ph indicator in litmus milk test

litmus
What is litmus in a base

blue
What is litmus in a acid

red
What enzyme do we test for in a litmus milk test

lactase
e.coli turn when positive turn the solid red in what test

litmus milk
Strep. lact. is positive when you see

whit bottom with red ring
what enzyme to we test for in the catalase test

catalase
If catalase is there what do you see

there will be bubbling of O2 gas
What is the reagent used in a catalase test

H2O2 (hydrogen peroxide)
Saccaharomyes cerevisiae Sac. cer.

employes yeast

Author

trsmith

81532

Card Set

Lab test 1

Description

micro biology

Updated

2011-04-25T03:08:33Z

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