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Lab test 1
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What is the primary stain in gram staining
Crystal violet
What is the mordant in gram staining
Grams iodine
What is the decolorizer in gram staining95/ethanol
95% ethanol
What is the counter stain in gram staining
safranin
E.coli is gram neg
Red rod
Bac. Cer is gram pos
Blue rod
Staphylococcus aureus is gram pos
Blue grape like cluster of cocci
What stain is used in negative staining
Nigrosine
Negative stain bac. Cer. Looks like
Long rods
Negative stain sar. Lut. Looks
Small cluster-cellular arrangements called tetrads
What is the backround in neg staining
Black
What is the primary stain in a capsule stain
1% crystal violet
What is the wash in a capsule stain
20 % Copper sulfate
What does strep. Lactose look like in a capsule stain
Purple diplococci in white capsule, purple backround
What is the primary stain in a acid- fast stain
Carbolfuchsin
What is the mordant in acid- fast stain
Steam heat
What is the decolorizer in acid-fast stain
Acid alcohol =HCL+95%ethanol
What is the counter stain in acid-fast staining
Methylene blue
What bacterium is mycobacterium smegmatis
Fushia rods
What is the primary stain in spore staining
Malichite green
What is the mordant in spore staining
Steam heat
What is the decolorizer in spore staining
Water
What is the counterstain in spore staining
Safranin
What does bac.cer. Look like in a spore stain
Green spores on red rods / endospores
seratia marcescen in a pure culture is
Red
In a pure culture method what color is sarcina lutea
Canary yellow
Pure culture method petri plate, cac. cer looks
billowey
Thioglycollate Broth is used to
take ou O2 gas
What does Thioglycollate broth grow
anearobes and gaculitative bacteria
What gasius requirerment is clostridium sporogenes
anaerobic - tube
What gasius requirerment is escheria coli
facualtative
What gasius requirerment is Sarc. lut
aerobic
What gasius requirerment is bas sub
aerobic
What is used in the wright's tube test
cotton ball, 10% NaOH(sodium Hydroxide) and Pyrogallic acid is the acid used
Describe Psuedomonas eruginose
has a fruity odor and turns agar green due to green water soluble pigment
What bedia is used in to test mobility
motility test agar
What is the PH indicator in sugar fermentation
Phenal red
Phenal red in a neutral
red
phenal red in a base
red
phenal red in a acid
yellow
phenol red in a alkaline
yellow
What is the small tube inside the test tube called
Durham tube
What enzyme do we look for in starch hydrolysis test
Starch Hydrolase
What is the regeant used in starch hydrolysis test
iodine
What is the media used in starch hydroliysis test
starch agar
Bacillius subt. is positive in starch agar
shows a light halo around bacteia and purple background
Staph aur. is negative in starch agar
shows purple throughout the petri dish
What enzyme is tested for in Urea Hydrolysis
urease
What media is used in urea Hydrolysis test
urea broth
What is the PH indicator in urea hydroloysis
phenol red
Prot. vulg. is positive for urease when it
changes to cerise in color
What is the substrat in litmus milk
litmus milk
What is the ph indicator in litmus milk test
litmus
What is litmus in a base
blue
What is litmus in a acid
red
What enzyme do we test for in a litmus milk test
lactase
e.coli turn when positive turn the solid red in what test
litmus milk
Strep. lact. is positive when you see
whit bottom with red ring
what enzyme to we test for in the catalase test
catalase
If catalase is there what do you see
there will be bubbling of O2 gas
What is the reagent used in a catalase test
H2O2 (hydrogen peroxide)
Saccaharomyes cerevisiae Sac. cer.
employes yeast
Author
trsmith
ID
81532
Card Set
Lab test 1
Description
micro biology
Updated
2011-04-25T03:08:33Z
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