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Smallest unit of life:
Cell
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How do surface area, volume, and size affect a cell's efficiency?
- Lower SA:Vol. ratio: big size and low efficiency
- Higher SA:Vol. ratio: small size, higher efficiency
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Plant exclusive features
- Central vacuole
- Chloroplasts
- Cell wall
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Animal exclusive features
- Lysosome
- Centriole
- flagella (sometimes)
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Eukaryotes
big complex structure, has nucleus and organelles
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Prokaryotes
small, simple structure, no membrane-bound organelles.
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Special features of Prokaryote:
- nucleoid
- cell wall
- flagella
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Nucleoid
clump of nucleic acids
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Nucleus
holds genetic material (nucleic acids)
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Rough ER
studded with ribosomes that synthesize protein (rough= ribosomes)
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Smooth ER
no ribosomes; makes lipids
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Golgi Apparatus
modifies proteins and lipids, ships them within the cell and exports outside the cell.
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Vesicle
membrane covered transport packages
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Lysosome
contain lytic enzymes that digest large molecules, recycle cellular resources, and can program cell death
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Peroxisomes
similar to lysosomes, but in plants
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Vacuole
large membranous sacs for storage and breakdown
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Central Vacuole
plants only; stores water, nutrients, pigments and waste
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Chloroplasts
plants only; converts light energy to chemical energy (photosynthesis), green due to chlorophyll
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Mitochondria
converts energy from food to usable energy for cell (cellular respiration)
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Cytoskeliton
maintain cell shape, assists in movement
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Cytoplasm
gell-like filling of cell
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Fluid-Mosaic model
- 1) phospholipid bilayer: fluidy portion
- 2) cholesterol: balances out fluidity of phospholipid bilayer
- 3) membrane porteins: mosaic part
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Phospholipid Bilayer
Hydrophilic heads (phosphate) and hydrophobic tails (fatty acid tails); is selectively permeable
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What does cholesterol do for the membrane?
stiffens the membrane
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Passive transport
- doesn't require ATP
- goes with concentration gradient (high to low)
- can require a carrier protein (facilitated transport)
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Active transport
- requires ATP
- goes against concentration gradient (low to high)
- requires a protein powered by ATP
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Diffusion
movement of small nonpolar molecules
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Osmosis
movement of water
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Facilitated transport
movement of ions, amino acids, glucose, and water
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Aquaporins
proteins that help transport water
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Tonicity
ability of a solution to cause a cell to gain or lose water
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Isotonic
concentration in and of the cell is the same
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Hypotonic
concentration outside of cell is greater than inside; waters flows into cell
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Hypertonic
concentration inside cell is greater than outside; water flows out of cell
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Membrane-Assisted transport
movement of BIG molecules through vesicles
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Exocytosis
membrane-assisted transport out of cell
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Endocytosis
membrane-assisted transport into cell
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Energy
the capacity to do work
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Kinetic energy
energy of motion; mechanical
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Potential energy
stored energy; chemical
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First law of Thermodynamics
energy can't be created or destroyed, but it can be changed from one form to another
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Second law of Thermodynamics
when energy is changed from one form to another, there is a loss of usable energy; waste energy goes to increase disorder
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Metabolism
sum of cellular chemical reactions
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Exergonic reation
release energy out
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Endergonic reaction
absorbe energy in
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Substrate
reactants that bind to enzymes
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Energy of activation
energy needed to start a reaction
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Characteristics of Enzymes
- enzymes are specific for 1 substrate/reaction
- 1:1 match, enzymes named after substrate
- are recyclable
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pH affect on enzymes
enzymes are pH sensitive and will only work in certain pH's
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Cofactors/coenzymes
help enzymes work better
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Inhibitor (enzyme)
makes enzyme work worse
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Competitive inhibitor
blocks area where substrate should go on enzyme
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Noncompetitive inhibitor
binds to enzyme at the allosteric site
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Feedback inhibition
the end product of a pathway inhibits the pathway's first enzyme
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How many kinds of substrates can bind to an enzyme?
1
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Chemical equation for photosynthesis
- 6CO2 + 6H2O --(light)--> C6H12O6 + 6O2
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Stomata
little pores on underside of leaf to help CO2 to enter the plant
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Mesophyll Tissue
fleshy part of leaft
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Thylakoids
discs in chloroplasts filled with chlorophyll
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Stroma
fluid filling in chloroplast
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What to processes make up photosynthesis?
Light reaction and Calvin cycle
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NADPH
electron carrier and coenzyme
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Reduction reaction
gain electrons
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Oxidation reaction
lose electrons
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LEO says GER
- Lose Electron: Oxidation
- Gain Electron: Reduction
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G3P
3 carbon sugar used to make glucose (1/2 of glucose)
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ATP Synthase
enzyme that makes ATP
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Light Reaction steps
- 1) capture light energy w/ chlorophyll which excites and electron (released)
- 2) water splits to replace lost electron and produces oxygen
- 3) excited electron passed down ETC
- 4) create H+ gradient (active transport)
- 5) NADP+ accepts electrons to become NADPH
- 6) produce ATP
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Where does the Calvin Cycle occur?
Stroma
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Calvin cycle
- 1) incorporate/fix CO2
- 2) use up NADPH and ATP
- 3) make G3P
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Importance of NADPH and ATP
they connect the light reaction and Calvin cycle
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Photorespiration
a wasteful process that produces no ATP or sugars
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C4 plant
different arrangement of cells and different carbon molecules in Calvin cycle
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CAM plant
different schedule, only open stomata at night
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Anaerobic respiration
- provides rapid burst of ATP
- regenerates NAD+ for glycolysis
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Chemical equation for Cellular Respiration
C6H12O6 + 6O2 ----> 6CO2 + 6H2O
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Who does Cellular Respiration, plants or animals?
both
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All organisms have what organelle?
mitochondria
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3 Phases of Cellular Respiration and their places of occuence
- 1) Glycolysis: cytoplasm
- prep reaction
- 2) Citric Acid cycle: mitochondrion
- 3) ETC: inside inner mitochondrial membrane (fold)
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Process of Glycolysis
glucose splits in half ---> 2 pyruvates (3 carbon sugars) ----> make NADH, ATP (electron carrier)
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Prep reaction after Glycolysis
pyruvate ----> Acetyl-CoA (2 carbon molecule), then enters mitochondria
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Steps of the Citric Acid cycle
- 1) Acetyl-CoA enters cycle
- 2) Carbons gained, lost, and modified
- 3) Yeild ATP, NADH, FADH2
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Steps of ETC in Cellular Respiration
- 1) NADH oxidized ----> NAD+
- FADH2 oxidized ----> FAD+
- 2) electrons passed down ETC
- 3) create H+ gradient
- 4) O2 accepts electron ----> H2O
- 5) produce ATP (a lot!) couple H+ gradient w/ ATP synthase
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What electron carriers connect the 3 stages of Cellular Respiration?
NADH and FADH
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What step in Cellular Respiration makes the most ATP?
ETC
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Affects of Anaerobic Respiration
- no final electron acceptor in ETC
- NADH can't recycle back to NAD+
- glycolysis stops because NAD+ is required
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Where do these happen:
-Lactic acid fermentation
-Ethanol alcohol fermentation
- Lactic acid: muscle cells
- Ethanol: yeast cells
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