In What year was the first edition of The Guide published
When did the FDA administer the GLP regulations
AALAS was previously known as
Animal Care Panel founded in 1950 until 1967
What is the scientific name of the Syrian Hamster
What color is this hamster
What color is the Armenian Hamster and scientific name?
Cricetulus Migratorius Gray
What is the name of the European Hamster
Hamsters hibernate at what temp
5 degrees C or 41F
The hamster is resistant to what 2 things
pulmonary infection irradiation-syrian chinese
When must the female pregnant hamster be moved to another cage?
One week prior to and 10 days post parturition
Strains with High Prevalence of LSA
BALB/c- multicentric SCID-Thymic
DBA's are prone to these conditions
soft tissue mineralization mammary tumors ectromelia audiogenic seizures
Difference between male and female mice spleens
sexual Dimorphism between pituitary and adrenal glands
THis mouse is a natural reservoir for what zoonotic disease in dogs?
meriones unguiculatus use in research
ligation of one common carotid artery and cerebral infarction
Epilepsy nematode infections and anthelminthic resistance
moist nasal dermatitis caused by increased harderian porphyrin secretion in
Mastomys natalensis use in research
Infectious and zoonotic disease submaxillary salivargy glands are a rich source of nerve growth factor
Dental formula in Oryctologus Cuniculus
i2/1,c0/0,pm3/2, 2-3/3 x 2
Gestation in rabbits last how long?
Sexual maturity in rat
50+/- 10 days, female earlier than male
When do Does ovulate?
10-13 hr after copulation
What is the recommended ambient temp for a rabbit?
61-72 deg. F
Weaning for a kit is when?
"Animal" as defined by the PHS
any live, vertebrate animal (cold or warmblooded) used in research, training, or testing
any building, room, or area used for confinement, transport, or experiments including surgery
–if animals are kept there for more than 24 hrs. it is considered an animal facility
PHS Assurance for what duration
According to the PHS policy, what members must consititute an IACUC?
1. DVM with experience or expertise in LAM and science 2. practicing scientist with experience in animal research 3. Non-scientist 4. Non-affiliated member
According to the PHS policy, how many members must their be in the IACUC
In the cat, What hrmone is secreted by the placenta and the only pregnancy specific hormone?
The cat has what type of reproductive cycle?
seasonally polyestrous, induced ovulator
When is a cat in proestrus and estrus?
When does anestrus occur in the cat?
What is the minimum photoperiod that will induce reproductive cycling in the cat?
More than 10 hours, 14 hours with flourescent bulbs
Peak sexual activity in the queen is for what duration of life?
Peak sexual activity for the tomcat?
Blood type ___ tomcats should not be bred to type___ Queens
A toms should not be bred to type B queens
kittens should be observed for what congenital abnormality?
cleft palate which leads to aspiration peumonia
kittens are weaned when?
-at 3-4 weeks start eating solid food - fully weaned at 8-10 weeks, healthy ones at 600g at 6 wks
cat scratch disease etioogic agent
Cutaneous larval migrans
visceral larval migrans
Unicinaria and Ancylostoma toxocara leonina and toxocara cati
Toxoplasma Sporolation occurs in how many days?
1-5 days, therefore litter boxes must be cleaned daily (AWR)
FIV and HIV resemble each other how?
cell and tissue tropism antigenically distinct
cage space allotementcats < 4kgs
Produced by repeated (N=10) backcrossess of a whole chromosome onto an inbred strain
ex. C57BL/6-X DBA
Produced with 1 Hybrid then repeated backcrosses of nuclear DNA or genome (C3H recipient male) onto cytoplasm (mitocondria) of female inbred mouse (ex. DBA donor) for 9 generations (N10 total) Ex. C3H/HeJ-mt DBA
After Hybrid mating (F1xF1) then 20 F2 matings produce progeny with unique sets of genes from parental strains. Used for gene mapping with phenotypic markers.
Hybrid then two backcrosses (N2) to parental strain. Ex. CcB(N3) or CcB (for N2)
Genetically identical except at 1 locus ex. C57BL/6J- Apo
Heterozygosity acheived at 1 locus after intercross matings BALB/cAnNCrl-
Similar genotypes except for short part of gene segment BC onto inbred stran 10 times. Less than 5= incipient congenic (;) ex. C.B- w
Advanced Intercross Lines
Producing an F2 generation between two inbred strains and then intercrossng in each subsequent generation avoiding sibling matings
APO is an example of mouse ____?
is an example of a recessive allele
When creating a transgenic mouse by random insetion, the DNA is usually micronjected into where?
the male pronuclei
In bred, albino, lung adenomas common, spleen cells for hybridomas
AK, inbred, Leukemia high icidence, hair interior defect mutation
(hid), Thy 1.1
C, inbred, monoclonal antibiodies, Th2 based response, ulcerative blepharitis and periorbital abcessation, tumors later in life
CB, inbred, agouti, retinal deg and autoimmune thyroiditis (Hashimoto's Disease)
C3, agouti, LPS resistant only, alopecia areata, on atherogenic diet don't devlop disease, no MMTV but can develop mammary tumors, Pde6b
rd1 homozygous, hepatomas in males
B6, tumor and disease resistant, microphthalmia, hydrocephalus, preference for sweet tastes and alcohol, incisor malocclusion
B10, production for histocompatibility complex in congenics, dermatitis, hydrocephalus and eye abnormalities, preference for sweets and alcohol 30% incidence of lymphoma
D1, dilute brown non-agouti, high incidence of mammary tumors and hearing loss at 10 mos., used in rheumatoid arthritis and nephritis
D2, first inbred strain, intolerance to morphie and alcohol, audiogenic seizures -used to test anticonvulsants
SJL (Swiss Jim Lambert)
J, inbred, albino, EAE, pelvic girdle muscular dystrophy, get Hodgkins reticular cell sarcomas, rd, males very aggressive
SW, albino, inbred, EAE, mammary tumor, rd, nephrogenic DI with age
white bellied agouti chinchilla, pink eyed chinchilla, inbred, used for ES for Knockout mice, incidence of testicular teratomas
Black Kaliss, Black, inbred Used for background strain of LeprProgressive hearing loss by 3 mo db Lep ob
Brown, inbred, atherosclerotic diet, vestibular and hearing deficits, progressive hearing loss at 7 weeks, fast learner
Leaden gray, inbred, diet induced atherosclerosis and gall stones, EAE, Hodgkins like Lymphoma, 3 months hearing loss
FVB/N (Friend Virus B virus)
albino, large pronuclei, asthma like hyperresponsiveness, squamous cell carcinomas induction High activity, anxiety, basal body temp, hypertermia, leukemia after 1 year, retinal deg.
CD-1, CFW or Swiss Webster (SW)- ideal pseudopregnant recipients for black and agoutis, NMRI, CF-1, Black Swiss-nuture fosters well, ideal pseudopreg recipient for albino, testing germline for 129 ES and C57 chimeras
Coronavirus causes what as an animal model?
myocarditis and cardiomyopathy for congestive heart failure
Main clostridial species implicaqted in the diarrhea and enterophathy of rabbits
C. spiriforme (g+) anaerobe
This anatomical structure in the rabbit serves as lymphoid tissue and is a thickening at the ilealcecal junction
This anatomical feature in the rabbit allows digesta through the cecum from the ileum
Dutch belted rabbits with this disease develop lesions similar to what human disease?
0157:H7 EHEC, hemolytic Uremic Syndrome (HUS)
What two areas should be cultured for diagnosis of P. multocida in the rabbit?
tympanic bullae and nasal cavity
Rabbit (Shope) Fibroma, virus type, natural reservoir, transmission
Poxvirus, sylvilagus floridanus- cottontail, insect vectors
Myxomatosis, virus, transmission
pox (leporipoxvirus), DNA virus, mosquitos
New World Monkey Characterisitics
New World Monkey
D3 in diet, D2 is not bioavailable Vitamin C
Old World Monkey
Vitamin C only
Old World monkey nose
New World Monkey tail
pseudo or prehensile
Dental formula of Cebidae (New World)
2 (4/2/6/2)= 36
The only nocturnal simian primate
These nhp have a toilet claw on second digit of foot for grooming
Callitrichidae (nwp) and old worlds dental formula
2(4/2/6/4) and 2( 4/2/4/6) = 36
These types of nhp have menstrual cycles
OWP/ catrrhine primates
These new world primates often twin and are permanent chimeras
callatrichids: marmosets (callatrhix jaccus) tamarins (saguinus oedipus)
Gestation of a callitrichid
Saimiri spp. (nwp, platyrrhine, cebidae, saimiriane) males under go this before winter_____
Females do this for their young ____
fatting allomother seasonally
This cebidae species spread herpes to others
Black and Gold Howler monkey scientific name
Olive Baboon scientific name
Papio hamadryas anubis
Saguinus oedipus is what common name and prone to what after cold stress?
Animal model for what?
Cotton Top Tamarin, chronic colitis colonic adenocarcinoma
What nhp has cheekpouches (family name, ow or nwp?)
Callathrix Jacchus are used as models for what?
EAE- multiple sclerosis Parkinsons disease idiopathic hemochromatosis
African green monkey
chlorcebus (cercopithicus) aethiops blue balls!
Cacajao culvus, short tail, long GI tract
In which nhps does karyotype matter?
Saimiri and Aotus
These nhp are old world primates and have a rhinarium
Prosimians have what characteristics?
toilet claw 2 part manible 36 teeth epithelial chorial placenta moist rhinarium with fissured fixed upper lip
Model for male pattern baldness
macacca arctoides (stump tailed macaque)
Lee boot effect
Spontaneous pseudopregnancy or diestrus when females are housed together
estrul synchrony when exposed to male's urine
When exposed to strange male's urine, pregnancy is terminated
Stop having sex with the partner you're with and desire for novel partner and promiscuity
Diploid chromosome number in rat
Ambient Temp according to Guide for rodents
Ambient temp according to guide for Cat, Dog, NHP
Rabbit ambient temp in Guide
Space reqt (Guide) for Rats <100 gm
Height reqt for rats of any weight
7 sq in
Rats up to 200 gm (Guide)
23 sq in
Rats up to 300 g (guide)
29 sq in
Rats up to 400 g (guide)
40 sq in
Rats up to 500 g (Guide)
60 sq in
Rats >500 g (Guide)
> 70 sq in
Rat female + litter
124 sq in
Cobblestone Lung appearance
This ricketssial parasite is transmitted via Polyplax spinulosa
This bacterium causes splenomegaly and septicemia in the rat
This organism is not transmitted easily by dirty bedding and occurs in conjunction with M. pulmonis (cobblestone lung)
CAR bacillus- wathrin starry or methenamin silver
This causes Hemorrhagic fever with renal syndrome in humans (HFRS), viral family?
Hantavirus in rats, Bunyaviridae
Toxoplasma gondii can be distinguished from E. cuniculi by what stains?
Toxo: H&E E. Cuniculi: Gram
These 2 strains of rats often get hydronephrosis
Gunn- Single dominant gene BN- polygenic
This rat strain has a high incidence of interstitial Leydig cell tumors?
Wehn culturing for Pseudotuberculosis (etiology?) what organ should be chosen as the best site?
Corneybacterium kutscheri, submaxillary/cervical lymph nodes
Gestation in the rat
This mutant rat strain gets diabetes Insipidus (mode of inheritance?)
Brattleboro, autosomal recessive
These 2 rat strains are models for prostatic adenocarcinoma
This rat strain is a model for transplantable Morris hepatoma
This rat shrain is a model for myeloma
This rat strain is a model for mononuclear cell leukemia
This rat strain is a model for multiple sclerosis, and autoimmune disease
Mouse diploid chromosome number
Genes that control coat color
Aa, Agouti, nonagouti; Bb, Black brown; Cc, Color, albino; Dd, non Dilute, dilute
Intercross is between 2 _____zygotes
Taurine is ___ in the urine, Tryptophan is ___ in the urine
what has an attenuating effect on Salmonella infection?
_______ _____ causes fatal dermatitis in nude mice
Streptococcus are in Lancefield group __
Polyplax serrata is the what?
Arthropod vector for what?
mouse louse Eperythrozoon coccoides
MRM requires what when growing?
THis mycoplasma sp causes rolling diesease in mice
Mousepox susceptible strains
A, BALB/c, C3H, DBA/1, DBA/2, (ABCD)
This rat louse may be a vector for Ectromelia
MCMV lesions in what organ
cervical salivary gland
LDHV is a ___virus
LDVH is the
#1 contaminant of tumor lines and cultures and causes increase in LDH for what strain of mice?
MCMV and MTV are ___virus
What genus and species amplifies and is susceptible to LCMV?
LCMV is a ____virus
PVM is in the family of
EDIM is a rotavirus in what family?
Sendai is a ___virus and causes what change in bronchial epithelium
paramyxovirus, squamous metaplasia
Oily Hair Effect
Reovirus 3 (Orthoreovirus, reoviridae)
MHV's hallmark lesion is
MEV is a ___virus
Mouse Adenovirus has 2 strains
FL Mad-1-fatal K 87 (MAd-2)- non path
Aspircularis tetraptera eggs requires how long to be infective
6 days (S. oblevata- 6 hours shorter lifecycle 15)
Most common ectoparasite of the lab mouse
Myocoptes musculinus boxing glove
list the RNA mouse viruses
LCMV LDV MHV PVM SV MEV Reo Rota MNV (Little lambs May Play So Mom May Run Right)
LCMV is a _ virus (genus)
MEV is a ____ virus
MEV is enveloped or non enveloped RNA virus
non enveloped (Rotavirus also)
C58 and AKR mice are susceptible to what virus, and why?
LDV have endogeonous MuLV and FV-1 homozygous
What strain of mice is susceptible to MHV infection?
How do you distinguish PVM from SV?
SV has squamous metaplasia of bronchial epithelium in the reparative stage
In what strains of mice is MNV lethal
STAT1 and ILabg def. mice
MHV acute death and wasting sydrome in which strain of mouse?
All of the mouse RNA viruses are _____ except which two?
Enveloped, only rota and MEV are nonenveloped and ds (reo)
The diabetic (db/db) and the obese Zucker (fa/fa) have mutations in the gene expressing what ?
The __ rat is susceptible to induction of experimental allergic encephalomyelitis while the ___rat is resistant
Lewis Brown Norway
Dry Bulb temp for rodents
Dry bulb temp for dogs cats and nhp