-
ELISA:
- (wells fixed w/ Ag)
- Add P Serum
- Wash
- Secondary Ab: Anti-Ig
- Wash
- Substrate for Color Change
-
Ag Capture ELISA:
- (wells fixed w/ Ab)
- Add P Ag
- Wash
- Ass known Ab + Conjugate
- Wash
- Substrate for color change
-
Western Blot for Ag:
- Electrophoresis
- Blot
- Probe w/ Ab + Conjugate
- Wash
- Add Substrate (color or radioactive)
-
Western Blot of Ab:
- Electrophoresis
- Blot
- Probe w/ Anti-Ig
- Wash
- Add Substrate (color or radioactive)
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