-
Cover the smear with crystal violet for 20 second
Gram staining
-
What is the mordent for gram staining
Gram's iodine
-
Decolorize the stain with 95 percent ethnal alcohol
Gram's staining
-
Cover the stain with safranin for 20 second
Gram staining
-
In gram staining bacillus cereus what color do rods stain
Blue
-
Is bacillus cereus gram pos or Neg
Pos
-
Is escherichia coli gram pos or gram neg
Neg
-
Is staphylococcus aureus gram pos or neg
Pos
-
In negative staining what stain is used
Nigrosine
-
Do you heat fix a negative stain
No
-
Place the edge of the clear slide into the nigrosine by backing it
- into the nigrosine and dragging it foward.
- Negative staining
-
Sarcina lutea negatve stain results
4 cocci, tefrad cellular arrangment
-
Bacillus cereus negative stain results are long rods
-
Cover the stain with 1percent aqueous solution of crystal violet
Capsual staining
-
Wash with 20percent copper sulfate
Capsual staining
-
Do you heat fix a capsular stain
No
-
Streptococcus lactis capsular stain results
Two nucleus in one cell (like an egg)
-
Enterobacter aerogenes
Rods longer than ecoli
-
What is the primary stain used for acid-fast staining
Carbol fuchsin
-
What is the mordent for acid fast staining
Steam heat
-
What is the decolorizer for acid fast staining
95percent ethenol alcohol
-
What is the counter stain for acid fast staining
Methylene blue
-
What are the results of mycobacterium smegmatis with a acid fast
- staining
- Red rods Blue cocci
-
What is the primary stain in spore staining malchite green
-
Cover the slide with malachite green a place slide atop a beaker of
- boiling water
- Spore staining
-
Counter stain the stain with safranin
Spore staining
-
What is the counter stain in spore staining
-
What is the result of spore staining escerichia coli and bacillus cereus
Red rods and green spores
-
To isolate a specific bacteria what method is used
Pure culture
-
Name two types of prue culture methods
Streak plate method and Pour plate method
-
What are the three types of gaseous requirements
Aerobic, anaerobic and facultative
-
what three methods are used to find the gaseous requirment of a
- bacteria
- Wright tube, thioglyollate and Petrie plate
-
Slightly loosen the lid of a tube of thiogycollate broth and boil it
- for 10 minutes, allowing the dissolved oxygen to be driven off.
- Thioglycollate broth method
-
Obtain a nutrient agar slant tubes, inoculate it with bacteria, place
- a cotton plug into the test tube about one- fourth inch from the top
- of the slant
- Wright's tube method
-
What acid is uesed in the wright's method
Pytogallic acid
-
What base is used in the wright's method
10 percent NaOH
-
Sterilize an inoculating loop and asepically streak one loopful of
- bacteria onto one side of a Petri plate
- Petri plate method
-
What is the gaseous requirment of escherichia coli
Facultative
-
What is the gaseous requirment of clostridium sporogenes
Anaerobic
-
What is the gaseous requirment of sarcina lutea
Aerobic
-
What is the gaseous requirment of bacillus subtilis
Aerobic
-
Does escherichia coli grow in thioglycollate broth method
Yes
-
Does escherichia coli grow in
- The wright's tube method
- Yes
-
Does escherichia coli grow in
-
Does clostridium sporogenes grow in thioglycollate broth method
Yes
-
Does clostridium sporogenes grow in
-
Does clostridium sporogenes grow in petri plate method
No
-
Does sarcina lutea grow in triogycollate broth method
No
-
Does sarcina lutea grow in wright's method
No
-
Does sarcina lutea grow in petri plate method
Yes
-
Does bacillus subtilis grow in the thioglycollate broth method
No
-
Does bacillus subtilis grow in the wright's tube method
No
-
Does bacillus subtilis grow in the petri plate method
Yes
-
What are the cultural characteristics of bacillus cereus
Flat, white, dry, motile, billowy, opaque
-
What are the cultural characteristics of escherichia coli
Raised, white, mucoid, motile, translucent
-
What are the cultural characteristics of proteus vulgaris
Flat, white, mucoid, motile, translucent
-
What are the cultural characteristics of staphylococcus aureus
Raised, white, mucoid, not motile, opaque
-
What is hanging drop slide method used for
To study motility
-
What is the motility test agar method used for
To test for motility
-
What are the results of motility test on proteus vulgaris
Motile
-
What are the results of motility test on pseudomonas aeruginosa
Motile
-
What are the results of motility test on escherichia coli
Motile
-
What are the results of motility test on sarcina lutea
Not motile
-
In sugar fermentation the results of dextrose and bacillus subtillis
Acid/no gas
-
In sugar fermentation the results of dextrose and escherichia coli
Acid/gas
-
In sugar fermentation the results of dextrose and staphylococcus aureus
Acid/no gas
-
In sugar fermentation the results of dextrose and alcaligenes
-
In sugar fermentation the results of lactose and bacillus subtillis
Base/no gas
-
In sugar fermentation the results of lactose and escherichia coli
Acid/gas
-
In sugar fermentation the results of lactose and staghylococcus aureus
Base/no gass
-
In sugar fermentation the results of lactose and alcaligenes viscolactis
Base/no gas
-
In sugar fermentation the results of maltose and bacillus sbtilis
Base/no gas
-
In sugar fermentation the results of maltose and escherichia coli
Acid/gas
-
In sugar fermentation the results of maltose and staphylococcus aureus
Base/no gas
-
In sugar fermentation the results of maltose and alcaligenes visclactis
Base/no gase
-
In sugar fermentation the results of saccharose and bacillus subtillis
Acid/no gas
-
In sugar fermentation the results of saccharose and escherichia coli
Base/no gas
-
In sugar fermentation the results of saccharose and staphylococcus
-
In sugar fermentation the results of saccharose and alcaligenes
-
In sugar fermentation the results of sucrose and bacillus subtilis
Acid/no gas
-
In sugar fermentation the results of sucrose and escherichia coli
Base/no gas
-
In sugar fermentation the results of sucrose and staphylococcus aureus
Base/no gas
-
In sugar fermentation the results of sucrose and alcaligenes viscolactis
Base/no gas
-
What is the reagent in the starch hydrolysis method
Iodine
-
What are the results of bacillus subtilis in the starch hydrolysis
- method
- Positive for starch hydrolace
-
What are the results of staphylococcus aureus in the starch hydrolysis
- method
- Negative for starch hydrolysis
-
What are the results bacillus subtilis in the urea hydrolysis method
Negative no urease
-
What are the results escherichia coli in the urea hydrolysis method
Negative no urease
-
What are the results proteus vulgaris in the urea hydrolysis method
Positive produces urease
-
What are the results staphylococcus aureus in the urea hydrolysis method
Negative no urease
-
The purpose of this exercise is to contrast this enzymatic ability
- between various bacteria
- Litmus milk method
-
What color is the base in the litmus milk method
Blue
-
What color is the acid in the litmus milk method
Red
-
What are the results of escherichia coli
- In the litmus milk method
- Turned pink, positive for enzyme lactase
-
What are the results of alcaligenes viscolactis In the litmus milk
- method
- Negative for enzyme lactase blue (produces ropey curd)
-
What are the results of streptococcus lactis In the litmus milk method
Positive for enzyme lactace (pink ring) round top (white clot bottom)
-
What are the results of proteus vulgaris In the litmus milk method
Negative for lactace (stayed blue) negative ropey curd
-
What are the effects of catalase on staphylococcus aureus
Positive
-
What are the effects of catalase on saccharomyces cerevisiae
Positive
-
What are the effects of catalase on eschericia Coli
-
What are the effects of catalase on streptococcus lactis
-
What are the effects of hydrogen sulfide on proteus vulgaris
- Positive for Sistine desulerace (enzyme)-enzyme, dark parcipitant
- (lead sulfide)-product
-
What are the effects of hydrogen sulfide on eschericia coli
Negative for Sistine desulferase
-
What do you add to nitrate broth when converting from nitrates to
- nitrites
- Sulfanilic acid and dimethyl alpha-napthalamine
-
What is nitrate reduction
- The enzymatic ability to convert nitrates to nitrites between various
- bacteria
-
What is the effect of nitrate reduction on proteus vulgaris
Positive for nitratase-enzyme
-
What is the effect of nitrate reduction on eschericia coli
Positive for nitratase
-
What is the effect of nitrate reduction on pseudomonas aeruginosa
Positive for nitratase w/zinc
-
What is the effect of nitrate reduction on staphylococcus aureus
Positive for nitratase w/zinc
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