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What are the 4 types of media
enriched, differrentia, selective and plate count
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What does enriched media mean
- It is fortified
- ex: brain, heart and fussion agar
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What does Differrentia media mean
- It is agar that posesses a ph indicator
- ex; indo agar and emb agar.
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what color does endo agar go to
Pink to purple
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What color does emb agar go to
purple to green sheen
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What is selective media
agar that posesses a growth inhibiter to reduce unwanted growth (highly selective)
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Give one example of a selective media
- Staph 20 agar (20% NaCl agar)
- halophilic-survives high salt
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SS agar stands for
Salmonella/shigella agar
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What is the inhibiting factor of SS agar
Penicillin
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What is plate count
Nutrient agar (all purpose media)
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Give 4 PH indicators
Phnalphthalein, Phenal Red, Methol Red and Bromthymal blue
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What color is phenalphthalein in an acid state
clear
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What is phenalphthalein in a base
pink (used in exlax)
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What is phenal red in a netral position
Red
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What is phenal red in a base
Red
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What is phenal red in a acid
yellow
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What color is Methal Red in a nutral state
Red
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What color is Methol Red in a base
orange
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What color is Methol Red in an acid
Red
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What color is Bromthymal blue in a nutral state
green
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What color is Bromthymal blue in a base
blue
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What color is Bromthymal blue in an acid
yellow
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What color is lipmus paper
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Ph meter
measured the concentration of hydrogin ions
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What are the factors needed to measure growth
- proper ph (most 6.8-7.2)
- proper neuirient
- proper temperature
- proper atmosheric condition (oxygen)
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what are the effects of temperature
- psychophiles (cold lovers)(5-20c opt 15)
- mosophiles 20-40c (opt 30-37c= 98.6)
- thermophiles Hot lovers (40-70c opt60c)
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How do we remove oz
- Thioglycolate broth (boil off oxygen)
- Wrights tube (acid + base> h20+salt
- hcl+NaOH>Nacl+H20
- H2-CO2 gerator
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How do we measur growth
- Turbitity,
- Cell mass,
- Nitrogen level,
- slide count
- plate count
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What is turditity
- Cloudyness- use a spectrophotometer neasures dead and live bacteria cells,
- used to make vaccines
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How do we use cell mass to measure growth.
- Grow cells,
- pour off the supernatant,
- dry the cell
- weigh cells
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How do we use Nitrogen level to measure growth
Nesler neagent + NH5. yellow-brown increase NH5-increaseyellow brown. More color more bactria
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