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Sterilization
Kills everything even endospores
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Disinfection
Kills vegetative pathogens, not endospores
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Antisepsis
Destroys/inhibits vegetative pathogens on living tissue
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Sanitization
- Mechanically removing microorganisms to reduce contamination to a safe level
- Think of food service
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Biocide (germicide)
Kills pathogenic microogranisms, not endospores
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Bacteriostatic
- Inhibits growth of bacteria Ex. cold temperature.
- static = inhibits doesn't kill
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Dessication & Hypertonic solutions
Both bacteriostatic
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Moist heat
spores 120, 80 vegetative, relative resistance 1.5x
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Radiation (x-ray) dosage
spores 0.4 Mrad, vegetative 0.1 Mrad, relative resistance 4x
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UV rays (exposure times)
spores 1.5h, vegetative 10min, relative resistance 9x
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Sterilizing gas (ethylene oxide)
spores 1,200 mg/l vegetative 700 mg/l relative resistance 1.7x
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Sporicidal liquid (2% glutaraldehyde)
spores 3h, vegetative 10 min, relative resistance 18x
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Dry Heat sterilization
Direct incineration or hot air sterilization in an oven at 170c for 2 hours
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The effect of cold (refrigeration)
0-7c (bacteristatic effect) Some bac can grow. Typically no path growth except Listeria monocytogenes (listerosis)
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Salmonella
Common food borne infection
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Campylobacter jejuni
Acute intestinal infection
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Coxiella burnetti
Q fever
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Cryptosporidium
Cryptospordiosis
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E.coli O157:H7 Yersiniosis
Yersiniosis enterocolitica
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