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Composition of Blood plasma
- H20
- nutrients
- waste products of metabolism
- inorganic salts
- Enzymes, hormones, vitamins
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Nutrients contained in the plasma
- plasma proteins
- glucose
- lipids
- amino acids
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what are the plasma proteins?
- albumin
- fibrinogen
- globulin
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plasma protein is mostly ___
albumin
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delta globulins are AKA
immunoglobulins
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maintains the osmotic pressure of the blood
albumin in the blood plasma
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globulins that function against infection and disease
immunoglobulins/antibodies
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these globulins include the glycoprotein and lipoproteins
alpha and beta globulins
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they transport oligosaccharide, lipids, steroids and hormones
alpha and beta globulins
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transports iron in the plasma
transferrin
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transports copper in the plasma
Ceruplasmin
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what are the waste products of metabolism
- NPN
- lactic acid
- ketone bodies
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what are the NPN's
- urea
- uric acid
- NH3
- creatinine
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name some ketone bodies present in the blood
- acetone
- dihydroxybutyric acid
- diacetic acid
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name inorganic salts present in the blood
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cells in the blood without nucleus
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body's defense against infection
WBC
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responsible for blood clotting
thrombocytes
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thrombocytes are AKA
platelets
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what is the blood volume?
8-8% of the total body weight
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color of deoxygenated blood
dark red/purplish
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color of oxygenated blood
bright red
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Specific gravity of the blood
1.045-1.075
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density of whole blood
1.054-1.060g/ml
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density of plasma
1.024-1.028g/ml
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identify the organic constituents of the blood
- carbohydrates
- protein
- cholesterol
- NPN
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the only sugar found in blood plasma
glucose
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end product of protein catabolism
urea
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end product of purine metabolism
uric acid
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Test for Carbohydrates
Benedict's test
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Test for protein
- Millon's Test
- Hopkin Cole's Test
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Test for Albumin
serum + saturated salt
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Difference between albumin and globulin:soluble in water
albumin
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Difference between albumin and globulin:totally insoluble in saturated (NH4)2PO4
albumin
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Difference between albumin and globulin:partially soluble in saturated (NH4)2PO4
globulin
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Difference between albumin and globulin:insoluble in water
globulin
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Difference between albumin and globulin:insoluble in saturated NaCl
globulin
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Difference between albumin and globulin:soluble in saturated NaCl
albumin
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Difference between albumin and globulin:soluble in diluted neutral salt solution
both albumin and globulin
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Difference between albumin and globulin:coagulated by heating
both albumin and globulin
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Test for cholesterol
Lieberman Buchard Test
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positive result for Benedict's test
reddish brown solution after heating
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positive result for Millon's test
flesh to red precipitate
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positive result for Hopkin Cole's test
violet solution
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the positive result of Millon's test is due to ___
tyrosine component
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the positive result of Hopkin Cole's test is due to ___
Tryptophan
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Serum + sat (NH4)2PO4 =
white precipitate
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Serum + sat (NH4)2PO4 + NaCl =
white precipitate lessens
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positive result for Lieberman Buchard Test
greenish solution
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the residue in the test for cholesterol is extrcted using
CHCl3
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what reagents were added to cholesterol in the Liebarman Buchard test?
acetic anhydrate and H2SO4
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reagent for Chloride determination
AgNO3
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reagent for Phosphate determination
ammonium molybdate
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reagent for irondetermination
NH4SCN
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serum + AgNO3 =
white precipitate (AgCl)
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blood heated -->ashash+HCl--> filteredfiltrate + NH4SCN =
red color (FeSCN)
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responsible for the red color of the blood
heme
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blood + H2O + stoke reagent
dark red
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Inorganic macrominerals in blood
- Chlorides
- Phosphate
- Sodium
- Potassium
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primary anion of the extracellular fluid
chloride
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primary anion of the intracellular fluid
phosphate ions
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component of gastric HCl
Chlorides
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serves as important function in the transport of O2 and CO2 in the blood
chlorides
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condition wherein there's a lower than normal serum chloride anion concentration
hypochloremia
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important in the acid-base balance of the body
phosphate
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anion important in the production of ATP in the body
phosphate
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condition wherein serum phosphate levels are low
hypoparathyroidism
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high serum levels of phosphate
hyperparathyroidism
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primary cation of the extracellular fluid
sodium
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primary cation of the intracellular fluid
potassium
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maintains the osmotic prssure of the extracellular fluid
sodium
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ion that controls water retention in tissue spaces
sodium
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ion that helps maintain blood pressure
sodium
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low sodium ion concentration
hyponatremia
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high sodium ion concentration
hypernatremia
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principal cation of the intracellular fluid
potassium
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maintains osmotic pressure of the cells
potassium
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maintains the electric potential of the cells
potassium
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maintains the size of the cells
potassium
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low serum levels of potassium
hypokalemia
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high serum levels of potassium
hyperkalemia
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ions found in bones and teeth
calcium
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necessary for certain oxidative enzyme
copper
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buffers of the blood
- bicarbonate buffers
- phosphate buffers
- hemoglobin buffers
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acid-base disorders
- metabolic and respiratory acidosis
- metabolic and respiratory alkalosis
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low pH, low HCO3, low pCO2
metabolic acidosis
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low pH, high HCO3, high pCO2
respiratory acidosis
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high pH, high HCO3, high pCO2
metabolic alkalosis
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high pH, low HCO3, low pCO2
respiratory alkalosis
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filtrate from blood
urine
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urine is the medium excretionof:
- salts
- acids
- bases
- waste products like urea, creatinine, uric acid, sulfates
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responsible for the color of urine
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decrease flow of urine
oliguria
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oliguria may occur during (2)
high fever and certain kidney disease
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total lack of urine excretion
anuria
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anuria occurs when:
there's extensive kidney damage
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greater amount of urine excretion than the normal
polyuria
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polyuria occurs during:
excessive intake of waterorpathological conditions like diabetic patients
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a measure of the concentration of solute and the concentration power and diluting power of the kidney
specific gravity of the urine
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equipment used in determining specific gravity of urine
urinometer
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identify what test:urine + picric acid + NaOH
test for creatine
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identify what test:urine----> NaCl crystals
test for chlorides
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identify what test:urine----> NaCl crystals
test for chlorides
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identify what test:Urine + HCl
Test for carbonates
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identify what test:Urine + HCl + BaCl2
Test for sulfates
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produced in the body from arginine, methionine and glycine
creatinine
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condition in which abnormal amount of creatinine occurs in the urine
creatinuria
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comprises about 1/2 of the total solids in urine
urea
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increased output of ___ is seen in patients with gouty arthritis
uric acid
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other organic compounds in the urine
- amino acids
- allantoin
- hipporic acid
- urobilin
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low NaCl output is seen during (2)
fever and nephritis
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high level of output is seen in certain bone diseases and hyperparathyroidism
phosphates
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low levels are found in hypoparathyroidism
phosphates
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produced from the hydrolysis of urea
NH3
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Other ions found in urine
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presence of glucose in urine
Glycosuria
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presence of ketone bodies in urine
ketonuria
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presence of protein in urine
proteinuriaor albuminuria
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normally present in the stool but not in urine
bile acids
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reagent for glucose test
benedict's solution
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reagent for ketose test
(NH4)2SO4andsodium nitroprusside
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reagent for protein test
HAc + NaCl
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reagent for bile acid test
sulfur powder
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normal urine + benedict's reagent
blue with white ppt
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pathological urine + benedict's reagent
green w/ yellow to brown ppt
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normal urine + (NH4)2SO4+ Na nitroprusside
yellow solution
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pathological urine + (NH4)2SO4+ Na nitroprusside
red-purple ring (+)
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normal urine + HAc + NaCl =
bubble formation
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pathological urine + HAc + NaCl =
white gel precipitate
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presence of blood in urine
hematuria
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