ch.19

• DNA synthesis; 3' to 5' exonuclease (for proofreading); 5' to 3' exonuclease; removes and replaces RNA primers used in DNA replication (also functions in excision repair of damaged DNA).
• Bacteria Cells

• DNA synthesis; 3' to 5' exonuclease (for proofreading); used in synthesis of both DNA strands
• Bacteria Cells

• Nuclear DNA synthesis; forms complex with primase and begins DNA synthesis at the 3' end of RNA primers for both leading and lagging strands (also functions in DNA repair).
• Eukaryotes

• Mitochondrial DNA synthesis
• Eukaryotes

• Nuclear DNA synthesis; 3' to 5' exonuclease (for proofreading); involved in lagging and leading strand synthesis (also functions in DNA repair). They think DNA Pol epsilon does the same thing.
• Eukaryotes

• RNA synthesis; makes RNA oligonucleotides that are used as primers for DNA synthesis
• Both Eukaryotes and Bacteria

• Unwinds double-stranded DNA
• Both Eukaryotes and Bacteria

• Binds to single-stranded DNA; stabilizes strands of unwound DNA in an extended configuration that facilitates access by other proteins.
• Both Eukaryotes and Bacteria

• Makes covalent bonds to join together adjacent DNA strands, including the okazaki fragments in lagging strand DNA synthesis and the new and old DNA segments in excision repair of DNA.
• Both Eukaryotes and Bacteria

• Bind to origin of replication and initiate unwinding of DNA double helix
• Both Eukaryotes and Bacteria

• Using an integral RNA molecule as template, synthesizes DNA for extension of telomeres (repetitive sequence at the ends of chromosomal DNA)
• Eukaryotes

• 1. DNA Polymerase
• 2. Primers
• 3. Nucleotides

Enzymes that degrade nucleic acids (usually DNA) from one end. Unlike endonucleases that make internal cuts.