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Rapid identification methods have been developed which provide a large number of results from one inoculation. What example of this did we complete in lab?
Entertube II - Lab 14
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Enterotube II is divided into ___ compartments, each containing a different substrate in agar
12
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The substrates in an un-icocculated Enterotube II are ___ by adding a bacterial suspension
rehydrated
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The observation that some microbes ___ the growth of others was made as early as 1874
inhibited
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___ and others ovserved that infecting an animal with Pseudomonas aeruginosa protected the animal against Bacillus anthracis
Pasteur
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Antibiosis means ___ ___.
against life
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Alexander Fleming observed ___ around a mold (Penicillium) growth on a culture of staph
antibiosis
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A. Waksman isolated the antibiotic streptomycin, produced by an antinocymete. This antibiotic was effective against many bacteria that were not affected by ___.
penicillin
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Antimicrobial chemicals absorbed or used internally, whether natural (antibiotics) or synthetic, are called ___ ___.
chemotherapeutic agents
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The concentration of a chemotherapeutic agent at the edge of the zone of inhibition represents its ___ ___ ___.
minimum inhibitory concentration (MIC)
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___-___ agar allows the chemotherapeutic agent to diffuse freely.
Meuller-Hinton
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After making a lawn of bacteria to test certain antibiotics, how long should you let it stand before placing the antibiotic disks on it?
5 minutes
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What type of agar plate will be used for the hand washing lab?
BAP (2)
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Semmelweis established a policy for the medical students of hand washing with a chloride of lime solution that resulted in a drop in the death rate due to ___ ___ from 12% to 1.2% in one year.
puerperal sepsis
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Hospitals show hand washing rates as low as ___.
31%
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Microbes that are present only for days or weeks are referred to as ___ ___.
transient microbiota
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The upper respiratory system consists of the ___ & ___
nose and throat
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The lower respiratory system consists of the ___, ___, ___ ___, & ___.
larynx, trachea, bronchial tubes, and alveoli
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The lower respiratory tract is normally ___ because of the efficient functioning of the ciliary escalator
sterile
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The upper respiratory system is in contact with the air we breathe, which is ___ with microorganisms.
contaminated
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The throat is a moist, warm environment, allowing many ___ to establish residence
bacteria
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Species of many different genera such as staphylococcus, Meisseria, and Haemophilus can be found living as _ microbiota in the throat.
normal
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Despite the presence of potentially pathogenic bacteria in the upper respiratory system, the rate of infection is minimized by ___ ___.
microbial antagonism
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Certain microorganisms of the normal microbiota suppress the growth of other microorganisms through competition for nutrients and production of ___ ___.
inhibitory substances
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___ species are the predominant organisms in throat cultures, and some species are the major cause of bacterial sore throats ( acute pharyngitis).
Streptococcal
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Streptococci are identified by ___ ___, including hemolytic reactions and antigenic characteristics (Lancefield’s system).
biochemical characteristics
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___ ___ are based on hemolysins that are produced by streptococci while growing on blood agar.
Hemolytic reactions
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Blood agar is usually made with defibrinated ___ blood (5.0%), sodium chloride (0.5%) to minimize spontaneous ___, and nutrient agar.
sheep & hemolysis
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Complete hemolysis, giving a clear zone with a clean edge around the colony
Beta hemolysis
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Incomplete hemolysis, producing methemoglobin and a green, cloudy zone around the colony
Alpha hemolysis
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No hemolysis, and no change in the blood agar around the colony
Gamma hemolysis
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Strep that is Alpha or Gamma is normal ___ of the throat
microbiota
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Strep that is ___ hemolytic are frequent pathogens
beta
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The streptococci can be antigenically classified into ___ groups A through O by antigens in their cell walls.
Lancefield
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Over 90% of streptococcal infections are caused by beta hemolytic group A strep – these bacteria are assigned to the species _ ___, which are sensitive to the antibiotic bactitracin
S. pyogenes
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Other strep are resistant to bactitracin, so ___ is used as well to distinguish between the pathogenic S.pneumoniae and other alpha hemolytic strep
optochin
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In the throat culture, what area of the throat is being swabbed?
Glossopalatine arches (golden arches)
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The presence of bacteria in urine is not considered an indication of ___ ___ ___ unless there are 1000 bacteria of one species or 100 coliforms per milliliter of urine.
urinary tract infection
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Many infections of the urinary tract, such as ___ or ___, are caused by opportunistic pathogens and are related to fecal contamination of the urethra, and to medical procedures, such as catheterization.
cystitis or pyelonephritis
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Pseudomonas is a ___-___ aerobic rod
gram-negative
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Pseudomonas aeroginosa is commonly found in the ___ and other environments.
soil
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Pseudomonas aeruginosa infections are characterized by ___ ___ pus. This bacterium produces an extracellular, water-soluble pigment called pyocyanin (“blue pus”) that diffuses into its growth medium.
blue-green
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The most common reportable communicable disease in the US is ___, an STD.
gonorrhea
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To determine the total number of cfu/ml, you must divide the ___ of ___ by 0.00001
number of colonies
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In urine inoculation, what will be used in place of the metal inoculation loop?
Sterile 10 μl calibrated loop
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