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urinalysis
provides information of the state of the kidneys and the animal's ability to normally filter and excrete metabolites
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free flow (clean catch)
collect midstream, clean vulva or prepuce first, not good for bacterial culture
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cyctocentesis
needle into abdominal wall, aseptic technique, full bladder only and empty completely, urine cultures
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catheter
pass a rubber, plastic of metal catheter through urethra into bladder, do as asceptically as possible
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manual expression
obtain a midstream sample, clean vulva and prepuce, not food for bacterial cultures, and never do if obstructed or pregnant
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qualitative analysis
looking for the presence of cells
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quantitative analysis
looking for amounts of cells
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physical exam of urine (gross exam), chemical exam of urine, microscopic exam of urine
three portions of urinalysis
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12-20 ml or urine/lb/day
normal urine volume
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polyuria
increased volume of urine production. decreased specific gravity, increased water consumption
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pollakiuria
refers with frequent urination, often confused with polyuria by clients, indicates nephritis or diabetes
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oliguria
decreased volume of urine production
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anuria
complete absence of urine formation or elimination
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light amber or straw color
normal urine color in domestic animals
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pale yellow or colorless
renal disease, excess water intake, diabetes, pyometra
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dark yellow/yellow brown
decreased fluid intake, dehydration, fever (urine color)
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yellow brown, green yellow
bile pigments present (urine color)
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red or brown
if cloudy (hematuria) if translucent (hemoglobinuria), urine color
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brown or brown/black
hemoglobin and large amounts of bile, urine color
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green
drugs or bile pigments, urine color
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clear
should be normal if fresh (except horses), transparency or urine
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cloudy
may become cloudy after standing, may contain cellular debris
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urochromes
yellow color in urine is due to these pigments
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specific gravity
the density of a quantity of liquid as compared to distilled water, it measures dissolved solids
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1.025, 1.030
normal specific gravity for a dog and cat
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1.001, 1.060, 1.000
specific gravity or dilute, concentrated and distilled water
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refractometer
place one drop of urine on _____ and close lid and hold to the light
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urinometer
fill with urine and spin to read. read at the bottom of the meniscus. need large volume of urine
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reagent strips
dip strips into the urine and read. least reliable. compare color change
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alkaline urine (7-8.5)
herbivore urine pH
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acidic urine (6-7)
carnivore urine pH
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5.2-6.8
normal dog urine pH
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7-8.5
horse and cow normal urine pH
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6-8.5
pig normal urine pH
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4.8-7.5
man normal urine pH
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proteinuria
abnormal levels of protein in the urine, false positives found in alkaline urine
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glucosuria or glycosuria
glucose in the urine, normal urine is negative, acetaminophen will cause false positives
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acetone, acetoacetic acid, beta-hydroxybutyric acid
three types of ketones, normals animals have small amounts
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hematuria, hemoglobinuria, myoglobinuria
tests for blood in the urine detect these
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hematuria
presence of intact red blood cells in the urine, sign of disease in the urogenital tract
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hemoglobinuria
the presence of free hemoglobin in the urine (the separation of hemoglobin from the RBC)
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myoglobinuria
the presence of myoglobin in the urine, caused by muscle damage
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bilirubinuria
bilirubin in the urine, normal in dogs, can be detected with the Ictotest, false negative from exposure to sunlight
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5ml for 3-5 min at 1000-2000rpm
centrifuging the sample
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unstained
casts and crystals are best visualized on the _____ slide
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stained
cells, organisms and sperm are best visualized on the _____ slide
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2-3 RBCs/HPF
normal value of RBC in urine
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over 5 RBC/HPF
abnormal value of RBC in urine
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pyuria, leukocyturia
excessive WBC's found in the urine (pus in the urine)
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2-3 WBC's/HPF, greater than 5
normal and abnormal WBC's found in the urine
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squamous, transitional, renal
three types of epithelial cells
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0-1/HPF
transitional epithelial cells normal range, increased numbers suggest cystitis or pyelonephritis
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0-1/HPF
renal epithelial cells normal range, increased numbers indicate kidney disease
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casts
formed in the lumen of the distal and collecting tubules of the kidney, abnormals levels indicate a lesion on the renal tubules
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hyaline casts
highly refractile casts, indicates renal irritation, fever, exercise
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granular casts
most common type of cast, in large numbers can indicate acute nephritis and severe kidney damage
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epithelial casts
comprised of epithelial cells, can indicate acute nephritis and degeneration of the renal tubules
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leukocyte casts
casts combining WBC's, indicates inflammation in the renal tubules
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erythrocyte casts
appear deep yellow or orange, indicates bleeding from the kidney
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waxy casts
resemble hyaline casts but wider and with square ends, indicates chronic and severe degeneration of the renal tubules
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fatty casts
casts containing small droplets of fat that look like refractile bodies, indicates chronic and severe degeneration of the renal tubules
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brownian movement
bacteria will appear moving because of this type of movement, reported as few, moderate or many
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round (cocci) or rod (bacilli)
two shapes of bacteria
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yeast
often confused with RBC's, yeast are usually contaminants, rare
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crystalluria
presence of crystals in the urine, reported as occasional, moderate or many
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triple phosphate, 'coffin lids', struvites crystals
found in alkaline urine, 3-6 sides, colorless prisms
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amorphous phosphate and urates crystals
found in alkaline urine and are granular and found in acidic urine
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calcium oxylate crystals
small colorless envelope, usually with an X in the center, found in acid urine, seen in ethylene glycol toxicity (6-18 hours past ingestion)
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envelope shape, sometimes dumbell or ring formed
calcium oxylate shapes
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leucine crystals
small, round, sectioned centered (grapefruit), found in acidic urine
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tyrocine crystals
speculated and spindled, found in acidic urine
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cystine crystals
hexagon shaped (6 sides), may indicate renal tubular dysfunction, found in acidic urine
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parasite ova
seen in urine sediment of animals with urinary parasites or because of fecal contamination at time of collection
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capillaria plica, dioctophyma renale, stephanurus dentatus
types of parasite ova
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capillaria plica
bladder worm of dogs and cats
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dioctophyma renale
kidney worm of dogs
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stephanurus dentatus
kidney worm of pigs
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