Once a gene is cloned, its complete nucleotide sequence can be determined.
· The automated procedure for this is based on a technique called the __.
o This method was developed by British biochemist __.
dideoxyribonucleotide (or dideoxy, for short) chain termination method
Frederick Sanger
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Knowing the sequence of a gene allows researchers to compare it directly with gene in other species, where the function of the gene product may be known.
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If two genes from different species are quite similar in sequence, it is reasonable to suppose that their gene products perform similar functions. In this way, sequence comparisons provide clues to a gene’s function.