1. Researchers can make another kind of DNA library by starting with mRNA extracted from cells.
    The enzyme __(obtained from retroviruses) is used in vitro to make single-stranded DNA transcripts of the mRNA molecules.
    The __allows use of a short strand of __as a primer for the __.
    • reverse transcriptase
    • poly-A-tail
    • thymine (dT) deoxyribonucleotides
    • reverse transcriptase
  2. Following enzymatic degradation of the mRNA, a second DNA strand, complementary to the first, is synthesized by __.
    · The resulting double-stranded DNA is called __.
    • DNA polymerase
    • complementary DNA (cDNA)
  3. For creating a library, the __is modified by the addition of __at each end. Then the __is inserted into vector DNA in a manner similar to the insertion of genomic DNA fragments.
    • cDNA
    • restriction enzyme recognition sequences
    • cDNA
  4. The extracted mRNA is a mixture of all the mRNA molecules in the original cells transcribed from many different genes. Therefore, the __that are cloned make up a __containing a collection of genes.
    • cDNAs
    • cDNA library
  5. However, a __represents only part of the genome-only the subset of genes that were transcribed in the cells from which the mRNA was isolated.
    cDNA library
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