ID Exam 2

Morphology/Growth

Three periplasmic flagella (axial filaments);Not cultured in artificial media; Grown in rabbits.

Morphology/Growth

Endoflagella; Endemic and epidemic forms

Morphology/Growth7-20 endoflagella; Microaerophilic; Grown in BSK (Barbour-Stoenner-Kelley) broth

Antigenic Structure

No LPS; outer membrane lipoproteins; hyaluronidase; fibronectin

No extracellular virulence-producing toxins have been identified

Diagnosed using darkfield microscopy of lymph node aspirate

Immunoserology Confirmatory Tests include: FTA-ABS

Other tests include: TP-PA test

Antigenic Structure

Lipoprotein subsurface components; Outer surface proteins OspA, OspB, OspC

Diagnosed with IFA or ELISA immunoserology

Diagnosed with Wright stained thin film blood smear from febrile period

Antigenic Structure

VMP (Variable Outer Membrane Protein) undergoes changes in protein sequencing during episodic antigenic variation

Morphology/Growth

2 periplasmic flagella anchored at opposite ends

Stains well with silver impregnation methods

Obligate aerobes; grow well on semisolid media at 29C; Can survive in untreated water for months-to-years

Antigenic Structure

Specific outer membrane compounds that are associated with serotype-unique epitopes

No extracellular virulence products

Diagnosed with MAT (microscopic agglutination test)