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c17 lecture 3
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Taxonomy vs phylogeny
T: Phenetic organization, based on phenotypes
P: Based on evolutionary relationships
Polyphasic Taxonomy
Incorporates various phenetic and phylogenetic information, with 16S RNA analysis
Ferdinand Cohn
Father of Bacteriology
Sorted bacteria based on morphology
Robert Koch on bacterial species designation:
Bacteria that maintain the characteristics when cultured in the same conditions should be designated a species
Biological classification
D P C O F G S
How do we define species for prokaryotes?
- Prokaryotes lack sexual reproduction -> cant use interbreeding
- Collection of strains, housekeeping genes
Biovar
Differ biochemically and physiologically
Morphovar
Differ morphologically
Serovar
Differ in antigenic properties
Type strain
First strain isolated, used as a reference
Binomial system of nomenclature for microbes
Genus species
G. Species
Pathotypes
Pathogenic strain of bacteria after recieving new genetic information through HGT
Phenetic classification system for microbes
Based on as many characteristics as possible
Morphological
Physiological
Ecological
Molecular
Ecological characteristics of a microbe
Life cycle patterns
Symbiotic relationships
Pathogenic ability
Habitat
Growth Requirements
6 analysis methods of molecular taxonomy
1) Nucleic acid base composition
2) Nucleic acid hybridization
3) Nucleic acid sequencing
4) Genomic fingerprinting
5) Proteins
6) Fatty acids
G+C variation among prokaryotes
25-80%
G+C variation among a genus
<10%
Hybridization percentage and interpretation
0-25% = different genera
25-75% = same genus, different species
75-100% = same species
Most powerful and direct method for comparing genes and genomes?
Nucleic acid sequencing
3 main problems with phylogeny in microbes
1) Taxonomy not based on evolutive relationships
2) Asexual reproduction + HGT
3) No fossil record to support evolutive taxa
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Author
08yangwf
ID
69411
Card Set
c17 lecture 3
Description
c17
Updated
2011-02-28T03:18:48Z
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