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Microbial growth
increase in the # of cells of a population
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Generation time
aka doubling time: time it takes for a population to DOUBLE in #
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E. coli vs M. tuberculosis doubling time
20MIN vs. 12HRS
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Biofilms
many different microorgs that grow close togther.
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Example of biofilm
Dental plaque, scum in drain, layers on rocks in stream
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useful biofilms
Bioremediation to degrade harmful chems such as left over from OIL spills
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competition in biofilms
- when bacterias compete for nutrients, it may lead to one bac synthesizing TOXIC chems that kill another bac
- ie: mold synthesizes PENICILLIN which kills other bacteria
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cooperative behavior in biofilms
one type of bac may produce nutrients for others to use & grow
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Slime layer
- synth by bac in biofilms
- useful for adherance to solid surface
- protects against antibiotics & disinfectants
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closed vs open system for bac growth
in closed system, nutrient NOT renewed & waste NOT removed, open is opp
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sporulation
cells response to attempting to survive harmful conditions
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direct cell count
does NOT distinguish b/w live & dead cells, viable cell count DOES
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Sterilization
- process of removing or destroying ALL microorganisms & viruses
- will even remove ENDOSPORES
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Disinfectants
- germicides that are used to eliminate most pathogens, but may not kill some orgs and bac
- for use on inanimate objects, called DISINFECTANTS
- for use on animate objects (ie skin), called ANTISEPTICS
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Bacteriostatic
prevents growth of bacteria BUT does not kill the bac
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Chemical Disinfectants that breakdown Proteins
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Chemical Disinfectants that breakdown DNA
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Chemical Disinfectants that breakdown Cytoplasmic Membrane
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Disinfectant plate
uses bacterial growth on agar plate, with drops of disinfectant on plate. The WIDTH of the clear ring around the disinfectant shows how effective it is as a disinfectant
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