MM5

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An unbranched chain of ribonucleotides joined by 3’-5’ phosphodiester bonds

RNA
What subunits is RNAP made up of?
- 2 alphas
- beta
- beta prime
This part of RNAP identifies the promoter region

Sigma
This subunit of RNAP sticks to the DNA

Beta prime subunit
What does Rifampicin do?
- Inhibits Beta subunit
- stops bond formation
What does actinomycin do?
- Sticks to DNA
- Stops it from unwinding
RNA binds to DNA in this stage of transcription
- Initiation
- unwinds 17 bp
Sigma goes away and RNA is made in this stage of transcription

Elongation
The hairpin loop is made in this stage of transcription

Termination
Transcription and translation happen at the same time in these guys

prokaryotes
How many types of RNAP are in prokaryotes?

1
These types of RNAP are in eukaryotes
- 1-3
- + mitochondrial
This type of RNAP makes the primary transcript

RNAP II
Adding a piece of 7-methyl-guanosine to the 5’ end of mRNA is called this

5' capping
This enzyme does the 5' capping

Guanyltransferase
What does the 5' cap do?
- protects
- guides
- starts translation
Adding a couple hundred Adenines to the 3’ end of mRNA is called this

Poly-A tailing
The sequence of mRNA signals the whole “A-tailing”
- Polyadenylation sequence
- AAUAAA
What does the Poly A tail do?
- Stabalizes
- Helps it get out of nucleus
Removing the introns is called this

Splicing
This machine does the actual splicing

Splicosome
What is a splicosome made up of?

snRNA and proteins
These two things help make diverse amount of proteins from limited genes
- Alternative splicing
- RNA editing
shoving a STOP codon into the mRNA by surprise is called this

RNA editing

Author

Bobopudge

68862

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MM5

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Transcription

Updated

2011-02-25T14:00:48Z

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