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An unbranched chain of ribonucleotides joined by 3’-5’ phosphodiester bonds
RNA
What subunits is RNAP made up of?
2 alphas
beta
beta prime
This part of RNAP identifies the promoter region
Sigma
This subunit of RNAP sticks to the DNA
Beta prime subunit
What does Rifampicin do?
Inhibits Beta subunit
stops bond formation
What does actinomycin do?
Sticks to DNA
Stops it from unwinding
RNA binds to DNA in this stage of transcription
Initiation
unwinds 17 bp
Sigma goes away and RNA is made in this stage of transcription
Elongation
The hairpin loop is made in this stage of transcription
Termination
Transcription and translation happen at the same time in these guys
prokaryotes
How many types of RNAP are in prokaryotes?
1
These types of RNAP are in eukaryotes
1-3
+ mitochondrial
This type of RNAP makes the primary transcript
RNAP II
Adding a piece of 7-methyl-guanosine to the 5’ end of mRNA is called this
5' capping
This enzyme does the 5' capping
Guanyltransferase
What does the 5' cap do?
protects
guides
starts translation
Adding a couple hundred Adenines to the 3’ end of mRNA is called this
Poly-A tailing
The sequence of mRNA signals the whole “A-tailing”
Polyadenylation sequence
AAUAAA
What does the Poly A tail do?
Stabalizes
Helps it get out of nucleus
Removing the introns is called this
Splicing
This machine does the actual splicing
Splicosome
What is a splicosome made up of?
snRNA and proteins
These two things help make diverse amount of proteins from limited genes
Alternative
splicing
RNA
editing
shoving a STOP codon into the mRNA by surprise is called this
RNA editing
Author
Bobopudge
ID
68862
Card Set
MM5
Description
Transcription
Updated
2011-02-25T14:00:48Z
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