Two Test Molecular

  1. What is a mutation?
    A mistake in the cells genetic material
  2. How is a wiled type DNA altered?
    • Substitution
    • Deletion
    • Insertion
    • Duplication
  3. What causes Mutations?
  4. What are the two ways to combat mutation?
    • Reversion- healing (rare)
    • DNA repair mechanism- restoring damaged DNA (mismatch repair, excision repair)
  5. Through which cells are mutations inherited, and which cells are not?
    • Germ line- inherited
    • Somatic- not
  6. What two things can human disorders be?
    • Autosomal chromosome disorder (Homozygous recessive disorder)
    • Sex-linked disorders
  7. What is gene therapy?
    replacing a defected gene with a good copy of the gene
  8. What are the methods of delivery for gene therapy?
    • Plasmids
    • Adenovirus (DNA)
    • Retrovirus (RNA)
    • Particle bombardment
    • DNA
  9. What are the two ways of doing molecular fingerprinting?
    PCR and RFLP
  10. What is the procedure for RFLP?
    • RE digest
    • Electrophoresis
    • Southern blotting
    • Visualizing by autoradiograph
  11. How does PCR work for DNA fingerprinting?
    Amplify DNA fragment showing hyper variable region and discriminate the probe
Card Set
Two Test Molecular
Two Test (CH 12,13,18)