Two Test Molecular

What is a mutation?

A mistake in the cells genetic material
How is a wiled type DNA altered?
- Substitution
- Deletion
- Insertion
- Duplication
What causes Mutations?

Mutagen
What are the two ways to combat mutation?
- Reversion- healing (rare)
- DNA repair mechanism- restoring damaged DNA (mismatch repair, excision repair)
Through which cells are mutations inherited, and which cells are not?
- Germ line- inherited
- Somatic- not
What two things can human disorders be?
- Autosomal chromosome disorder (Homozygous recessive disorder)
- Sex-linked disorders
What is gene therapy?

replacing a defected gene with a good copy of the gene
What are the methods of delivery for gene therapy?
- Plasmids
- Adenovirus (DNA)
- Retrovirus (RNA)
- Particle bombardment
- DNA
What are the two ways of doing molecular fingerprinting?

PCR and RFLP
What is the procedure for RFLP?
- RE digest
- Electrophoresis
- Southern blotting
- Visualizing by autoradiograph
How does PCR work for DNA fingerprinting?

Amplify DNA fragment showing hyper variable region and discriminate the probe

Author

icimmy

67540

Card Set

Two Test Molecular

Description

Two Test (CH 12,13,18)

Updated

2011-02-20T00:49:54Z

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