Enzyme kinetics/inhibitors

Michaelis-Menten eqn
- V₀ = Vmax [S]
- K_m + [S]
Assumptions of MM eqn
- -Rate is V₀ --> ignore k₄
- -Single S & ES
- -Constant [ES]
- -[S]>>>[E]
Linear form of MM eqn

1/V₀ = (K_m/V_max)x(1/[S]) + 1/V_max
Ki
- [E][I]
- [EI]
Competitive I
- same Vmax
- higher Km
Non-competitive I
- lower V_max
- same K_m
- (binds E & ES equally)
Mixed I - preference for E
- lower V_max
- raise K_m
- (lowers k1 more than k2+k3)
Mixed I - preference for ES
- lower Vmax
- lower Km
- (lowers k2+k3 more than k1)
Uncompetitive I
- lower V_max
- lower K_m
- (to same extent --> all lines on lineweaver-burke plot same slope)

Author

bryan69604

66892

Card Set

Enzyme kinetics/inhibitors

Description

Michaelis-menten enzymes, effect of reversible inhibitors on Km/Vmax

Updated

2011-02-17T02:04:47Z

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