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Automated Cell Counts
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Who invented 1st automated cell counter?
Walter Coulter
Types of Hematology Analyzers
Particle Cell Counter (Electrical Impedance)
Optical Cell Counter (Optical Light Scattering Scanner)
Radio Frequency (RF) Resistance
Electrical Impedance
Cells do not conduct electricity.
Current is broken every time a cells is passed through electrodes.
RF Resistance
High frequency electromagnetic current flows between 2 electrodes.
Cell wall acts as a conductor to gauge size based on pulse.
Similar to Impedance but higher frequency.
Hydrodynamic
Directs Diluted Cells through quartz flow cell in single file.
Laser light directed at the flow cell is scattered in all directions.
Photo-detectors at different angles detect scattered light and create a countable pulse.
Low angle light scatter.
2-3
o
= cell size and volume
High Angle Light Scatter
5-15
o
= internal complexity
Right Angle Light Scatter
90
o
Side Scatter
Relates to internal components.
VCS Technology
Volume
Conductivity
Scatter
Volume is measured with... (VCS)
Using low frequency current.
Conductivity measured with....(VCF)
High frequency current measures changes in conduction.
Light Scatter (VCF)
Laser light used to differentiate WBCs based on surface shape and reflectability.
Impedence/VCS Technology Analyzer
Breckman-Coulter
Impedance/Hydrodynamic Focusing Analyzer
Roche-Sysmex
Optical Scanner/Impedance Analyzer
Abbott Cell Dyn
What is measured in a Automated CBC
RBC
WBC
Platelet Count
RBC Indices
Platelet Indices
5 Part Diff- Segs, eos, basos, lymphs, mosos
Reticulocyte count with newest types
Sweep Flow Technology eleminates what?
Double cell Counting
RBC Threshold Limit
>36 fl
Platelet Threshold Limit
2-20 fl
WBC Threshold Limit
>35 fl
Cell Dyn Laser
Argon
Histogram Left to Right
Lymphs ->Monos -> Grans
A (ABCD)
Monos
B (ABCD)
Neutrophils
C (ABCD)
Eosinophils
D (ABCD)
Lymphocytes
Author
moses1424
ID
65361
Card Set
Automated Cell Counts
Description
Automated Cell Counts
Updated
2011-02-10T07:07:27Z
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