Biochem oxidative phosphorylation

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Inhibitors of Complex I in ETC?
- Amobarbital
- Rotenone
- Piericidin A
Inhibitors of Complex II
- TTFA
- Carboxin & Malonate
Inhibitor of Complex III

Antimycin A
Inhibitor of Complex IV
- Cyanide
- CO
- Sodium Azide
- Hydrogen Sulfide
Uncoupling agents
- Aspirin(salicylic acid)
- 2,4 DNP
Purpose of uncoupling agents?

Thermogenic agents
Oligomycin?

Blocks ATP synthase
How do 2,4 DNP and Aspirin cause uncoupling between ETC and phosphorylation?

by making the inner mitochondrial membrane leaky, allowing H+ to diffuse into the matrix without going thru the ATP synthase
Atractyloside

Blocks ATP/ADP transporter in the inner mitochondrial membrane
What are two ways of transporting NADH into the inner mitochondrial membrane?
- 1.reducing DHAP to glycerol 3 phosphate which then enters matrix and reduces FAD
- 2. Oxaloacetate is reduced to malate which then enters matrix and reduces NAD+
Which complexes pump H+ into the intermembranous space?

I, III, IV
Whats the rate determining step of TCA cycle?

isocitrate dehydrogenase
Whats the rate determining step of glycolysis?

Phosphofructokinase-1 (PFK-1)
Whats the rate determining step of Gluconeogenesis

Fructose 1,6 bisphosphotase
What blocks Complex I?
- Amytal
- Rotenone
What blocks complex III

Actimyocin A
What blocks Complex IV
- CN
- CO
- H2S
- NAN3
What blocks ATP synthase?

Oligomycin
What are 2 uncoupling agents?
- 2,4-DNP
- Aspirin
Whats the only lipid component of the ETC?

Coenzyme Q ---Ubiquinone
How many protons are pumped out per a pair of electrons that go thru the ETC?

10
How many protons are pumped out per a pair of electrons when complex one is omitted?

6
Which part of the ATP synthase exhibits ATP synthase activity?

F1

Author

pszurnicki

64144

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Biochem oxidative phosphorylation

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Biochem oxidative phosphorylation

Updated

2011-02-07T17:11:35Z

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