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Bacteria
- Bacteriology
- micro meter
- 1x10^-6
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Viruses
- Virology
- nm (1x10^-9)
- can be seen with electron microscope
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Techniques to isolate & culture microorganisms
- 1.) isolation
- 2.) Sterilization
- 3.) Culture in artificial media
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fungi
- mushrooms
- penicillin & yeast
- used for making ber & wine
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Algae
- photosynthesis
- produse over 50% of oxygen
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cytology
cell characteristics/content
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physiology
function of growth for making disease
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ecology
tolerable temperatures for microorganism growth
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taxonomy
- classifications
- Kingdom, Phylum, Class, Order, Family, Genus, Species
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bioremediation
process of microorganisms to degrade waste
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Robert Hooke
- discovered microscope/lens
- did not see living thing
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Antonio Van Leeuwenhoek
1st to see living thing with microscope
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spontaneous generation
belief that life could orginate from non-living matter
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Francesco Redi
- disproved spontaneous generation
- - maggot was unable to grow on meat if meat was covered with gauze
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Schwann
- disproved spontaneous theory
- allowed air to enter flask after passing through heated tube
- sterile wool
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Louis Pasteur
- demonstrated that microorganisms were present in air
- - applied heat to flask bending neck and trapping microorganisms in air
- -25C optimum temp
- showed disease of silkworms was caused by protozoans
- developed vaccines for chicken cholera, anthrax, and rabies
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Fermentation
production of alcohol by breakdown of carbohydrates
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Agostino Bass
showed that silkworm disease was caused by fungus
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Charles Chamberland
identified virus as disease-causing agents
ex. (TMV) Tobacco Mosaic Virus
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Edward Jenner
vaccination procedure to protect from smallpox
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Joseph Lister
- phenol (carbolic acid) to disinfect wounds
- first antiseptic in surgery
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Robert Koch
1st to classify bacteria microorganism specificity
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Koch's Postulates
1.) etiological - causative agent must be present in all affected organisms
2.) agent - isolated from cultured in pure form
3.) cultured agent - introduced to healthy agent && cause same disease
4.) same causative agent - must be able to be removed from newly infected agent and cause disease
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Virulent strains not cultured in artificial media
- syphilis - Treponema pallidum
- leprosy - Mycobacterium
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Gelatin
- melts @ 28 degrees
- bacteria hydrolyze
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agar
- not digested by most bacteria
- melts @ 100 degreesC
- used in 2% solid media
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Swan neck flask
Pasteur used to disprove spontaneous generation
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culture
growth of bacteria in solid or liquid artificial media
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solid artificial media
separates mixtures of bacteria
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liquid artificial media
never used to separate mixtures of bacteria
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non-pathogenicity
do not cause disease
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avirulent (non-virulent)
do not cause disease
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Pathogenicity
ability to make diease
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virulence
degree of pathogenicity
opposite of attenuation
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attenuation
ability of virus to change under lab conditions to become less virulent (harmless)
damage in way disease can't be produced
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Pasteurization
application of temperatures to kill microorganism
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immunity
protection from a disease due to inoculation
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Tyndallization
boiling more than once to destroy resistant forms of bacteria endospores
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pure culture
culture that contains only ont type of microorganism
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Vaccination
inoculation to form immunity to a disease
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cell-mediated immunity
engulf bacteria and digest
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humoral mediated immunity
produce to kill bacteria neutralize w/liquid
ex. attenuated
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passive immunity
transsfer of antibodies from parent to offspring
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Active immunity
The production of antibodies against a specific agent by the immune system
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