What is the RNA polymerase made up up in prokaryotes?
2 alpha, one beta and one beta prime plus a sigma factor
When does sigma factor leave?
What topoisomerases are present during prokaryotic transcription?
Topo I and II
Which termination, rho dependent or independent uses palindromic GC rich then AT rich sequences to form a loop which dissociates the RNA polymerase?
What happens in rho dependent termination?
rho factor recognizes termination sequence upon which unwindins the nascent RNA molecule from the template strands terminating transcription
binds beta subunit of RNA polymerase preventing initiation of transcription
Intercalates between DNA preventing it from being transcribed
RNA pol II
What 3 factor bind to the TATA box which help to anchor DNA pol II?
TBP, TAF and TFIID
What TF bind to GC and CAAT sequences?
What are promoter proximal upstream elements
How is termination different for each of the eukaryotic RNA polymerases?
RNA pol I- polymerase specific termination factor
RNAII- coupled to a sequence that polyadenylates 3'
RNA-III- simiar to rho independent termination
what is the order after transcripton of capping,cleavage polyadenylation, splicing?
the above mentioned
What is the sequence of splicing?
2'-OH of the branch site attacks 5'-P of donor site
3'-OH of donor site joins 5'-p of acceptor site
Forms a Lariat in the process
ab against snRNP
beta thalasemia results from?