Microbiology Review Questions #1

This direct smear is from a urethral discharge on a male; how would you report it out?

This direct smear is from a urethral discharge on a male. What is the likely organism?

This chocolate plate was incubate in carbon dioxide. What is the term used for organisms that need increased carbon dioxide to grow?

What is the selective plate that can be used for isolation of Neisseria gonorrhoeae & Neisseria meningitidis?

What would be the expected oxidase test result if the organism is a Neisseria or Moraxella?

Is it more likely Moraxella catarrhalis or Neisseria gonorrhoeae is growing on this plate?

What is the term for Moraxella catarrhalis' nonfermentation of sugars?

What tests are positive for Moraxella catarrhalis?

Would you do a Kirby-Bauer susceptibility test or a beta lactamase for Moraxella catarrhalis?

From the results of these CTA sugars, what organism is this likely to be?

What is the term used for the presence of Neisseria meningitidis in the blood stream?

What would be the appearance of the CTA sugar tubes if the caps had been loose and the tubes were in increased carbon dioxide?

What member of the Streptococcaceae family will give a positive for both bile esculin agar and 6.5% NaCl broth?

What other test is positive for Enterococcus spp. other than Bile esculin and 6.5% NaCl?

What organism(s) would give a positive Bile esculin and negative 6.5% NaCl broth?

What members of the Streptococcaceae family will give a positive PYR test?

What organisms would give a positive esculin and positive PYR?

What organisms would give a positive esculin and a negative PYR?

What is the name of this test?

What organism produces this definitive arrow when close to Staphylococcus aureus (ie. Camp +) ?

What would be the expected results for Streptococcus agalactiae for PYR and Esculin?

What organism will give a positive hippurate test?

If we isolate Streptococcus agalactiae from a spinal fluid, what is the likely age of the patient?

What would be the expected Bacitracin susceptibility for Streptococcus agalactiae?

Describe the type of hemolysis seen with Streptococcus agalactiae.

Which streptococci is susceptible to bacitracin?

What Micrococcaceae family member is susceptible to bacitracin?

What type of hemolysis is present?
Are these colonies more suggestive of Group A, Group B, or Streptococcus pneumoniae?

What is the chemical compound found in the Optochin disk?

What other rapid test (other than optochin) can be done to identify Streptococcus pneumoniae?

How do you detect penicillin resistance for Streptococcus pneumoniae?

What should be the depth of the Mueller Hinton agar?

What organism is growing on this Kirby-Bauer susceptibility test?

Why is the depth of the Mueller Hinton agar important?

What are you reporting out with the "E-test"?

What are advantages of the "E-test"?

If an organism produces beta-lactamase, what groups of drugs does it have resistance to?

What organisms would you do a beta lactamase test for?

Which genus of the Micrococcaceae family is sensitive to bacitracin?

What are the characteristics that put an organism in the Micrococcaceae family?

What would be the result for bacitracin sensitivity for Staphylococcus epidermidis?

This is the catalase test. What is the reagent used for this test?

The catalase test is used to differentiate which gram-positive cocci?

What is the problem with performing a catalase test on blood agar or accidentally picking up blood agar with the colony?

What is the most likely type of hemolysis seen with Staphylococcus aureus?

What identification tests are positive for Staphylococcus aureus?

What is the result expected for novobiocin susceptibility for Staphylococcus saprophyticus?

What patient population is more likely to have urinary tract infections due to Staphylococcus saprophyticus?

Which organisms would give this Gram stain morphology from a specimen?

How would you perform a tube coagulase test?

Which organism(s) produce "sulfa granules" in the exudate from the infection?

If you gram stain the yellow flecks of "sulfa granules" what would be the gram stain reaction and morphology of the organism(s)?

Between Nocardia and Actinomyces,
which of the organisms is aerobic and which is anaerobic?

The lower picture is a Gram stain and the top picture is a modified acid stain of the same organism. What branching gram positive rod is acid fast?

What type of specimens might contain Nocardia?

What organism causes black lesion like these?

Is Bacillus anthracis beta hemolytic on blood agar?

What is the Gram stain morphology of Bacillus anthracis?

What is the lecithinase result on Egg Yolk agar for Bacillus anthracis?

If you had a nonhemolytic colony on blood that was motile, could you rule out Bacillus anthracis?

This is Tinsdale Agar that is used for what organism?

What other selective media can be used for Corynebacterium diphtheriae?

What is the name of the test used to determine if Corynebacterium diphtheriae produces the toxin that causes the disease?

What would be the appearance of the motility in a wet prep for Listeria monocytogenes?

What would be the CAMP test result for Listeria monocytogenes?

This Gram-positive rod is faintly beta hemolytic on blood agar and may look like Group B streptococci; what is it?

What type of food products may result in food poisoning due to Listeria monocytogenes?

How would you describe the smell of Haemophilus influenzae?

What media would support the growth of Haemophilus influenzea?

Starting at the top left quadrant: X only
Top right: V only
Bottom right: XV combined
Bottom left: Horse Blood agar

What organism is this?

What would be the expected growth reactions for Haemophilus influenzae?

What other test would be positive for H.parainfluenzae & H.parahaemolyticus?

What is the term used to describe colonies growing around other colonies that provide V factor?

What organisms can provide V factor?

What organisms will only grow on sheep blood agar if V factor is provided by another organism?

How is sheep blood different from horse or rabbit blood when growing Haemophilus spp. ?

If this was identified as "mercury drop" colonies on Bordet' Gengou, what organism is it likely to be?

What type of specimen is collected for Bordetella pertussis?

The picture at the right is a heart at autopsy. The arrow is pointing to infected heart valves. What is the term used for this type of infection?

The HACEK group all cause endocarditis. What organisms belong to the HACEK group?

What are the common characteristics of the HACEK group?

Which one of these organisms line up on Gram stain as rosette, stick-like forms?

If the organism is from a dog bite or a cat-licking a wound, what is the likely organism?

Does Pasteurella multocida grow on MacConkey?

What is the Gram stain morphology of Pasteurella multocida?

What are the catalase and oxidase reactions for Pasteurella multocida?

If this blood culture from an individual who ate unpasteurized goat cheese, what Brucella organism is it likely to be?

If this blood culture from an individual who raised beefalos, what Brucella organism is it likely to be?

What is the name of the Brucella organism associated with pigs?

What is the biosafety levels for positive cultures for Brucella?

This is a culture on Selective Buffered Charcoal Yeast Extract agar. What organism is likely to be isolated?