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Cells that take up DNA through their outer membranes are called _____ cells.
competent
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Competence is influenced by _____.
Growth stage, concentration of available DNA, and composition of medium; heat shock, chemical treatment
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The cell that receives DNA in a transformation is called the _______.
transformant
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When genes are transformed together, they are ______.
cotransformed
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The rate of cotransformation is _______ proportional to the distances between the genes which is useful for gene mapping.
inversely
-
Sometimes bacteria acquire DNA from eukaryotes in a process called ___________.
horizontal gene transfer
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The process of passing passing genetic information through reproduction is called ____________.
vertical gene transfer
-
A simple replicating structure made up of nucleic acid surrounded by a protein coat is called a _____.
virus
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__________ are viruses that infect bacteria, and have been used extensively for genetic studies.
Bacteriophages (phages)
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Virulent phages reproduce only through the _____ cycle and always kill their host.
lytic
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_________ phages can undergo either the lytic or the lysogenic cycle.
Temperate
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Phage DNA integrates into the bacterial chromosome and becomes a ________.
prophage
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When a bacteria lyses, the adjacent bacteria are infected which also lyse resulting in a clear patch called a _____.
plaque
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If a phage containing bacterial DNA transfers genes to another bacterium, recombination may take place and produce a _______.
transductant
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The rate of transduction is ___.
low
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The rate of cotransduction is _____ proportional ot the distances between genes.
inversely
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_____ transduction can only occur near an att site.
Specialized
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In specialized transduction, a phage called ________ can be produced resulting in either an unstable transductant or a stable transductant with a gal+ allele.
lambda gal defective
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_______ used the awesome power of phage genetics to make inferences about gene structure.
Seymour Benzer
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The sites of different mutations in the same gene can be mapped, referred to as _______.
intragenic mapping
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A _______ test indicates whether two mutations occur in the same or different genes.
complementation
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A _______ is a functional gene defined by a complementation test.
cistron
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_______ occurs when there is at least one wild-type copy of each gene, i.e. the mutations are in different genes.
Complementation
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What are the four basic types of chromosomes?
metacentric, submetacentric, acrocentric, telocentric
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A complete set of chromosomes possessed by an organism presented as an ordered image is called a _____.
karyotype
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What are three types of chromosome mutations?
rearrangements, aneuploids, and polyploids
-
What are four types of chromosomes rearrangements?
duplications, deletions, inversions, and translocations
-
When a single copy of a gene is not suf?cient to produce a wild-type phenotype, it is said to be a ______ gene.
haploinsufficient
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______ inversions do not include the centromere, while ______ do.
Paracentric, pericentric
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______ genes are nonviable in both peri and paracentric inversions.
-
A _______ involves the movement of genetic material between nonhomologous chromosomes.
translocation
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In a _______ translocation, there is a two-way exchange of segments between two chromosomes.
reciprocal
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In a _______ translocation, two long arms of chromosomes are combined creating a large metacentric chromosome.
Robertsonian
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In homologous pairing in translocation heterozygotes, the best option is ______ segregation.
alternate
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________ is an increase of a decrease in the number of individual chromosomes.
Aneuploidy
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Aneuploidy in meiosis ___ (I or II) results in 2 trisomic and two monosomic zygotes.
I
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Aneuploidy in meiosis ___ (I or II) results in 1 trisomic, 1 monosomic, and 2 normal (diploid) zygotes.
II
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In humans, ______ results in 44 chromosomes.
nullisomy
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In humans, monosomy results in how many chromosomes?
45
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In humans, trisomy results in how many chromosomes?
47
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In humans, ______ results in 48 chromosomes.
tetrasomy
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What mechanism for controlling gene dosage could account for viability of XXX females?
X-inactivation
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What creates a carrier in familial Down syndrome?
Robertsonian translocation
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____ is the presence of more than two sets of chromosomes.
Polyploidy
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____ are 5n in chromosome number.
Pentaploids
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_____ is a type of polyploidy where chromosome sets are from a single species.
Autopolyploidy
-
_____ is a type of polyploidy where chromosome sets are from different species.
Allopolyploidy
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The primary structure of DNA is the _____.
nucleotide sequence
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Secondary structure of DNA is the _____.
double-stranded helix.
-
One type of tertiary structure in DNA is ______, which takes place when the DNA helix is subjected to strain by being overwound or underwound.
supercoiling
-
Supercoiling is controlled by _______, enzymes that add or remove rotations from the DNA helix.
topoisomerases
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Two types of eukaryotic chromatin are the more common one, ____, and ____, which is present near centromeres and telomeres and along X-inactivated chromosomes.
Euchromatin, Heterochromatin
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Most abundant proteins in chromatin are ____ - small, positively charge proteins of 5 major types.
histones
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____ consist of DNA wrapped about 4 pairs of histones.
Nucleosomes.
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A _____ consists of a nucleosome plus the ____ histone acting as a clamp.
chromatosome, H1
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_____ chromosomes arise when repeated rounds of DNA replication take place without cell divisions in certain tissues in Drosophila.
Polytene
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Acetylation occurs when enzymes called _____ attach acetyl groups to lysine amino acids on the histone tails.
acetyltransferases
-
Centromeric sequences Serve as binding sites for _____ proteins that provide anchor sites for spindle fibers.
kinetochore
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____ was awarded the nobel prize for the discovery of how chromosomes are protected by telomeres and the enzyme telomerase.
Elizabeth Blackburn
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_____ are stabilizing sequences at the ends of chromosomes.
Telomeres
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______ proteins bind to the G-rich single-stranded sequence of telomeres.
POT (Protection Of Telomere)
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A structure called the _____ also functions in protecting the telomere from degradation.
t-loop
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Artificial chromosomes such as the YAC or BAC contain what there essential elements?
Centromere, pair of telomeres, origin of replication
-
Sequences present at one or a few times in the genome are called ___.
Unique-sequence DNA
-
Groups of related genes arising from duplication of unique-sequence DNA are called ___.
gene families
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The two types of repititive DNA are called ___ and ___ DNA.
moderately repetitive and highly repetitive
-
Two types of moderately repetitive DNA are called ____ and ___ .
tandem repeat sequences and interspersed sequences
-
_____ DNA sequences are sometimes called lite DNA and are often found at ___ and ___.
Highly repetitive, centromeres, telomeres
-
____ are mobile DNA sequences.
Transposable elements
-
______ result from staggered cuts made in the target DNA when a transposable element is inserted.
Flanking direct repeats
-
____ are present in many TEs are recognized by enzymes that catalyze the transposition.
Terminal inverted repeats
-
Terminal inverted repeats are ____ and ____ .
inverted and complementary
-
What are two classes/mechanisms of transposition?
Class I/DNA transposons, and Class II/Retrotransposons
-
What are two kinds of Class I/DNA transposons?
non-replicative and replicative
-
Class II/retrotransposons are always ____.
replicative
-
DNA transposons require an enzyme called ____ which is usually encoded by the TE.
transposase
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In replicative transposition, two DNA molecules are joined and the TE is replicated, producing a ____.
cointegrate
-
Retrotransposons use an ____ intermediate used to reverse transcribe back into DNA.
RNA
-
An enzyme called ____ is usually encoded by retrotransposons.
reverse transcriptase
-
Transposable elements were discovered in eukaryotes by _____.
Barbara McClintock
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Ds in maize was able to transpose nonautonomously using transposase from Ac elements. What is a possible reason Sleeping Beauty wasn't able to do this?
The inverted repeats were also mutated. Transposase REQUIRES inverted repeats.
-
What are three types of TEs in bacteria?
Insertion sequences, composite transposons, and noncomposite transposons
-
The simplest types of transposons in bacteria are the ___ and have 1-2 genes which encode ___.
insertion sequences, transposes(s)
-
A segment of DNA flanked by insertion sequences can transpose and is called a ____.
composite transposon
-
____ transposons do not have insertion sequences.
Noncomposite
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___ found in fruit flies have both a transposase and a repressor of transposition.
P elements
-
What were three models of proposed DNA replication?
conservative, dispersive, and semiconservative
-
____ performed an experiment to determine which of three models of DNA replication applied to E. col.
Meselson and Stahl
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____ replication of DNA takes place in circular DNA in bacteria.
Theta
-
_____ replication of DNA takes place in some viruses and in the F factor of E. coli.
rolling-circle
-
Eukaryotic genomes require multiple ____.
origins of replication
-
The process of DNA replicaiton includes many componets including (3):
a template, substrates (dNTPs), and enzymes
-
DNA synthesis always goes in the ____ direction.
5�-to-3�
-
Short fragments of DNA produced by discontinuous synthesis on the _____ strand are called ____.
lagging, Okazaki
-
Which mode/l of DNA replication does not have a lagging strand?
Rolling-circle model
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