What functions do enzymes play in the body?
They are catalysts.
They speed up the rate of chemical reactions in our body.
Enzymes are which type of substance?
What things can denature enzymes?
Strong Acid or base
What is a substrate?
The material acted upon by the enzyme.
How are enzymes named?
Name of substrate
Tells what kind of reaction and ends in -ase.
ex. glucose oxidase
Where are enzymes normally found?
In Intact cells
Alkaline phosphatase (ALP or ALKP) an diestive enzyme is active in what pH?
Work best at pH 10,
High pH optimum pH 10
Alkaline phosphatase increased in what kind of cancer?
SGOT / AST
Increased in what diseases?
SGPT / ALT a transferase enzyme is increased in only what disease?
CPK aka CK are found in which tissues?
What are the 3 isoenzymes and which tissue is each from?
CK1 - 2 BB chains from Brain and Lung
CK2 - MB chain from Heart
CK3 - MM chain from Muscle
What is important about LDH?
Present in almost all tissues.
Remains elevated longest in MI.
How are isoenzymes separated?
5 isoenzymes separated by Electrophoresis
Why are hemolyzed specimens not acceptable?
High concentration in RBC
Which 2 enzymes are ordered together to evaluate pancreatic functions?
Amylase and Lipase
Which test are in a cardic panel?
CPK or CK
Troponin I adnormal protein specific to heart
Troponin T adnormal protein specific to the heart and muscle, best predictor to ACS and MI.
Which test are in a liver profile?
GGT, GGTP or GT
Which is the most sensitive of the liver enzymes?
GGTP (gin and tonic)
Review for Chemistry Quiz on Enzymes