Plasmid Vectors (Slide 6)
Origin of Replication (OriP) used to maintain high copy number (often 100s in a cell) or low, as required
MCS (Multiple Cloning Site) has multiple RE sites that are present only at this site. Used as cloning sites for genes
As only 1% of cells are competent for plasmid transformation, selectable markers and selective media are used to facilitate screening
Other elements: Gene expression, shuttle vector features