Chapter 18 Bio LEcture

enzyme that catalyzes the joining of DNA fragments

a DNA molecule made in a lab from 2 or more different sources

the ends of the cut sites where complimentary sticky ends can attatch

to create an identical copy

a cloning process where DNA is cloned by inserting it into host cells

transformation of animal cells specifically

a host cell/organism that contains recombinant DNA

• allow growth of transgenic genes
• EX- genes resistant to antibiotics
• included in recumbant DNA sequence

replication unit

• a carrier DNA squence
• can enter host cell via this
• has its own origin of replication

any gene whose expression is easily observed

a collection of DNA fragments that together comprise the genome of an organism

• makes up a much smaller DNA library
• made from transcribed mRNA

a collection of cDNAs

a manipulation that inactivates the gene to keep it from transcribing and being translated

a technique to produce a knockout mutant

an unspecialized cell that divides and differenciates into specialized cells

a rare natural mechanism for inhibiting mRNA translation

• technique that allows for hybridization
• provides large arrays of sequences for hybridization expirements

the use of cell/organisms to produce useful materials

vectors that have all the characteristics of typical vectors along with other sequences needed for transgene

the production of medicine in farm animals/plants

the use of other organisms to remove contaminants from the environment