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Chapter 18 Bio LEcture
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DNA Ligase
enzyme that catalyzes the joining of DNA fragments
recombinant DNA
a DNA molecule made in a lab from 2 or more different sources
sticky ends
the ends of the cut sites where complimentary sticky ends can attatch
clone
to create an identical copy
transformation
a cloning process where DNA is cloned by inserting it into host cells
transfection
transformation of animal cells specifically
transgenic
a host cell/organism that contains recombinant DNA
selectable measure
allow growth of transgenic genes
EX- genes resistant to antibiotics
included in recumbant DNA sequence
replicon
replication unit
vector
a carrier DNA squence
can enter host cell via this
has its own origin of replication
reporter gene
any gene whose expression is easily observed
genomic library
a collection of DNA fragments that together comprise the genome of an organism
Complimentary DNA (cDNA)
makes up a much smaller DNA library
made from transcribed mRNA
cDNA library
a collection of cDNAs
knockout
a manipulation that inactivates the gene to keep it from transcribing and being translated
homologous recombination
a technique to produce a knockout mutant
stem cell
an unspecialized cell that divides and differenciates into specialized cells
RNA interface (RNAi)
a rare natural mechanism for inhibiting mRNA translation
DNA microarray
technique that allows for hybridization
provides large arrays of sequences for hybridization expirements
biotechnology
the use of cell/organisms to produce useful materials
expression vectors
vectors that have all the characteristics of typical vectors along with other sequences needed for transgene
pharming
the production of medicine in farm animals/plants
bioremediation
the use of other organisms to remove contaminants from the environment
Author
kickenbugg
ID
53890
Card Set
Chapter 18 Bio LEcture
Description
Ch 18 "Recombinant DNA and Biotechnology"
Updated
2010-12-07T02:29:53Z
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