1. coccus shape gram -
    Neisseria sp.
  2. coccus shape gram +
    do a catalase test
  3. catalase -
    preform Bile esculin hydrolysis
  4. catalase +
    preform MSA
  5. MSA growth
    1. mannitol fermentation
    • +. staphylococcus aureus
    • -. staphylococcus epidermidis
  6. Bile esculin hydrolysis
    • +. Enterococcus faecalis
    • -. Hemolysis
  7. Hemolysis
    Alpha hemolysis= Streptococcus pneumoniaBeta hemolysis= Streptococcus pyogenes
  8. Rod Shaped
    • Gram +
    • Gram -
  9. Gram - Rod
    Glucose and oxidase
  10. Glucose + Oxidase -
    Lactose test
  11. Lactose +, -
    • +. Methyl Red
    • -. Urea
  12. Urea
    • +. Proteus
    • -. H2S=+ Salmonella typhimurium
    • - Shigella flexneri
  13. Methy red
  14. Indole+,-
    • + E. coli
    • - H2S + Citrobacter freundil
    • - Citrobacter diversus
  15. Indole-
    • Motility
    • +Enterobacter aerogenes
    • -Klebsiella pneumonia
  16. Indole+
    K. oxytoca
  17. Acidic stain
    Basic stain
    Spore stain
    Acid Fast stain
    Capsule stain
    • .carries a negative charge
    • .carries a positive
    • .useful for staining Clostridium
    • .useful for staining Mycobacterium
    • .requires a basic stain and an acidic stain
  18. The order gram stain reagents are applied is:
    crystal violet, iodine, alcohol, safranin
  19. Complete this sentence: Negative staining procedures will:
    stain the background, not the cell.
  20. After being gram stained, gram positive bacteria are:
  21. Gram negative bacteria have:
    a thin layer of peptidoglycan in their cell wall
  22. The following is a differential stain:
    Gram stain
  23. The organism on the upper half of this MSA plate (surrounded by a yellow halo) is what organism?
    Staphylococcus aureus
  24. The medium best suited for differentiation of Streptococci is
    blood agar
  25. An isolate is a gram-positive coccus, catalase-negative, and optochin-resistant. It is most likely:
    viridans streptococci
  26. Some streptococcal hemolysins can completely lyse red blood cells resulting in a clear zone around colonies on blood agar. This reaction is called:
  27. Staphylococcus epidermidis is able to grow on MSA. It tolerates the high salt but is unable to ferment the sugar in the medium. The sugar is:
  28. The genus Neisseria is characterized by
    gram-negative diplococci with flattened adjacent sides
  29. The organism that causes strep throat is
    Streptococcus pyogenes
  30. The most widely used method to evaluate the effectiveness of antibiotics against specific bacteria is
    Kirby-Bauer method
  31. Which of the following genera include pathogens of the intestinal tract?
  32. What color is an uninoculated citrate tube?
  33. Which medium is best for differentiating Streptococci?
    Blood agar
  34. Which medium is best for differentiating Staphylococci?
    Mannitol Salt agar
  35. Which medium is best for differentiating enterics?
    Kligler Iron agar
  36. Which medium would you choose to test for indol production?
    pink or red
  37. A KIA tube is inoculated and grown for 24 hours. It has a black butt, red slant, and no cracks or fissures. This is indicative of:
    Glucose fermentation only
  38. Which of the following KIA reactions will you most likely observe after growing E. Coli for 24 hours in a KIA tube?
  39. The results of an IMViC series of tests is ++--. What color is at the surface of the SIM tube?
  40. The results of an IMViC series of test is --++. What color is the methyl red tube?
  41. A sample of dirt is boiled for in water 10 minutes to kill all vegetative cells. After cooling, you inoculate a TSA plate with a small amount of the dirt. Several colonies grow overnight. The colonies are most likely of which genus?
    • Clostridium
    • Bacillus
  42. Media in which some metabolic activity of an organism can be detected by inspection of the growth of the organism on the medium are referred to as:
  43. The carbohydrate in MSA is
  44. Beta-hemolytic, bacitracin sensitive streptococci are growing well on a culture medium. The most likely organism is:
    Staphylococcus pyogenes
  45. Chocolate agar is used for growing fastidious bacteria.
  46. Which of the following genera include pathogens of the intestinal tract?
  47. An isolate is a gram-positive coccus, catalase-negative, and optochin-susceptible. It is most likely
    Staphylococcus pneumoniae
  48. The mold hyphae you are viewing with your nouveau French microscope appears 156 times normal size. The ocular lens has a power of 12X. What is the power of the objective lens?
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