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Restriction enzymes
are bacterial proteins that cleave DNA at specific sequences.
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GAATTC
is a palindrome and a restriction site.
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List the importance of restriction enzymes in the treatment of type I & II diabetes.
- The human insulin gene is removed from human DNA and is subcloned into a bacterial plasmid.
- The bacteria then produce human insulin which can be used to treat type I & II diabetes.
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DNA is negatively charged.
True
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DNA is separated on gel electrophoresis based on:
molecular weight.
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Southern Blot
is used to identify DNA.
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Northern Blot
is used to identify RNA.
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Western Blot
is used to identify protein.
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A probe
- is defined as a single-stranded piece of DNA or RNA that is used to visualize complementary
- sequences within DNA.
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DNA sequencing
utilizes dideoxy nucleotides to sequence segments of DNA.
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PCR
stands for polymerase chain reaction and is a technique used to amplify DNA.
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Taq polymerase
is derived from the bacteria Thermus Aquaticus commonly found in hot springs.
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A gene knockout
is a technique used by researchers to knockout a particular gene of interest.
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A karyotype
- is a technique used to identify DNA chromosomal abnormalities. For example, trisomies,
- monosomies, and various other abnormalities can be detected by karyotypes.
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Cloning
is a technique used to create an exact copy of oneself.
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RNAi
- inhibitor RNAs
- is used to inhibit protein expression by inducing the degradation of mRNA.
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Dicer
is the enzyme responsible for cleavage of RNAi into siRNA.
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RISC
- stands for RNA induced silencing complex and is the enzyme responsible for cleaving the
- endogenous mRNA.
-
List the diseases currently being investigated for RNAi treatment.
- Macular Degeneration, Hypercholesterolemia, RSV, Hepatitis C, and various others are potential
- diseases being investigated as potential RNAi targets.
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