Microbiology iClicker Questions Exam 2

  1. If asked to sterilize a heat sensitive material, which would you use:

    B. ethylene oxide
  2. Which is incorrect:

    B. biofilms make bacteria more sensitive to antibiotics
  3. Which is not a chemical agent used to control microbes:

    D. filtration
  4. Household bleach is used as common disinfectant because it has:

    A. hypochlorite
  5. Resistance to antibiotics can result from the following mechanisms, except:

    A. underproduction of membrane components
  6. Which has the highest energy:

    A. phosphate anhydride bonds
  7. All energy in a living system is derived from
    - movement of electrons down an energy gradient
  8. If G˚’<0 then:
    - the reaction is exergonic and can occur spontaneously
  9. If organism A uses H2S as an electon donor and Fe3+ as electron acceptor AND organism B uses methanol as electron donor and O2 as electron acceptor, which probably produces the most energy:
    - Organism B (because O2 is electron acceptor)
  10. During glycolysis: R-P +ADP à ATP + R. What is this an example of:
    - substrate level phosphorylation
  11. What is the main purpose of fermentation?
    - Regeneration of NAD+
  12. What is the purpose of the Kreb’s Cycle?

    A. ALL
  13. If a cell is not working properly and can’t convert pyruvate to acetyl CoA, what would be the total ATP production?
    2 ATP
  14. Which is produced in the Kreb’s Cycle and used for biosynthesis of amino acids?

    A. Ketogluterate
  15. Which of these are carried by NADH and FADH2 and ultimately lead to ATP in the ETC?

    A. electrons & hydrogen ions
  16. Which is true for NADH?
    Has most negative Eo and greatest capacity to donate electrons.
  17. Place in the correct order that electrons are transferred:
    - NADH, FMN, Quinone, Cyt C, Cyt A, O2
  18. Which electron carriers associated with electron transport are lipid soluble?
    - Quinones
  19. When glucose is used as an energy source and O2 is the final electron acceptor, how many ATP are formed:

    C. 38
  20. How is ATP produced?
    - Harvesting the transmembrane gradient by ATP-synthase
  21. Oxidation reductase pairs X/XH2 and Y/YH2 have reduction potentials of -50mV and +75mV. This means an electron would most likely be removed from ______ to reduce _____.
    XH2, Y
  22. Aerobic respiration differs from anaerobic respiration in which respect?
    - The final electron acceptors are different
  23. A bacteria converts nitrate to nitrogen gas. This process is called:

    D. denitrification
  24. Which microbes use CO2 as an electron acceptor?

    D. a & b
  25. Which is not a final electron acceptor during anaerobic respiration?

    B. succinate
  26. Place these in order of highest energy yield to lowest:
    1. Respiration with O2 as electron acceptor
    2. Respiration with nitrate as electron acceptor
    3. Respiration with ferric ion as electron acceptor
    4. Catabolism of substrate linked to ion transport
    • 1. Respiration with O2 as electron acceptor
    • 2. Respiration with nitrate as electron acceptor
    • 3. Respiration with ferric ion as electron acceptor
    • 4. Catabolism of substrate linked to ion transport
  27. Which is incorrect for anoxygenic photosynthesis?

    D. The electron donor is H20
  28. The enzyme hydroxylamine oxidoreductase…
    - oxidizes NH2OH to NO2
  29. The enzyme nitrite oxidoreductase…
    - oxidizes NO2 to NO3
  30. In chemolithotrophic bacteria that use H2 as an electron donor, which is incorrect:
    - acetic acid is produced at the terminal oxidase
  31. In the nitrogen cycle, the final product of _____:

    A. nitrification is ammonia
  32. In autotrophic bacteria, CO2 is fixed by:

    A. a,b, &c
  33. Select the correct statements about nitrogenase:
    1. Nitrogenase-reduces cyanide, acetylene, and other triple bonded compounds
    2. Nitrogenase is transcriptionally regulated by oxygen, ammonia, and certain amino acids
    3. Nitrogenase catalyzes the denitrification of ammonia
    4. Nitrogenase is the terminal oxidase in anaerobic respiration
    1 & 2
  34. You dilute samples before plating in enrichment because:
    - you want to dilute out weed species that grow quickly but aren’t that abundant
  35. To eliminate the live-to-dead ratio of cells in sample, use:

    C. Bac Light
  36. Why are killed cell controls so important when using radioisotopes to measure microbial transformations?

    D. Because some transformations can occur abiotically and controls will help you asses those abiotic transformations
  37. Organism A converts cellulose to cellobiose. Organism B converts cellobiose to glucose. What is their relationship?
  38. Which of the following is not mutualism?

    A. Beggiatoa oxidation of H2O2
  39. Each band on a DGGE gel represents ____:

    D. A different phylotype/strain represented in the community DNA
  40. If you have a group of various species of microorganisms that can all oxidize sulfide in a given habitat, this is called a _____

    C. guild
  41. Where is microbe activity highest in soil?

    B. The rhizosphere
  42. Select the incorrect statement about biofilms.

    C. biofilms only cause medically related problems
  43. In one common symbiotic relationship one of the associates benefits from association. The other is either harmed or even killed. This form of symbiosis is:

    C. parasitic
  44. Which of the following would be a reason for the observation that generally microbes grow more slowly in nature than they do in laboratory culture?

    D. All of the above
  45. A microbe that lives at a depth of 5000m in the ocean would be

    E. more than one of the above, but not all
  46. Which of the following is an incorrect statement about pelagic and benthic microbes?

    D. Most benthic microbes are phototrophs
  47. Choose the correct statement(s) about endoliths.

    D. a,b,&c
  48. The use of microbes to degrade and detoxify pollutants is known as

    D. bioremediation
  49. In competition for nutrients and resources, bacteria may secrete bacteriocins which are:

    A. peptides that disrupt the cell membrane
  50. Which of the following is not difference between prokaryotes and eukaryotes?

    A. presence of nucleoid in eukaryotes
Card Set
Microbiology iClicker Questions Exam 2