Preparation and examination procedures 5.2

This is a poisonous chemical in polyvinyl alcohol and Schaudinn's fixative.

Formalin is used as a _________.

What are three examples of flamables used in the parasitology?

for direct collection of fecal specimens a ______ mouth plastic container should be used.

When performing O&P exam intestinal protozoa will be undetectale for at least one week after what procedure?

O&P exams should be delayed one week following what four medications?

What are five susbstances that may interfere with O&P exams?

explain intestinal parasite specimen collection.

explain amoebiasis specimen collection.

parasite specimens should be submitted immediately because these forms will die after 30 min.

This is the preservative of choice for parasite specimens, may be used for permanent slides.

true or false
formalin concentrations can be drawn from PVA-preserved specimens.

how long are specimens preserved with PVA stable for?

what are the two disadvantages of PVA?

This preservative is used for fresh stool and mucosal linings, adapted to inpatients.

What is the disadvantage of Schaudin's preservative.

This preservative is used in 10% concentrations, heat solution to disinfect.

what is the disadvantage of formalin preservative?

in MIF what stains and what preserves?

What are the two disadvantages of MIF.

This is done on all fecal specimens to ensure detection of even small numbers of organism.

This concentration technique allows eggs and cyst to float to surface. used to detect protozoan cysts and nematode ova.

This concentration technique contains zinc SO4, sugars, and NaCl, and a high spec. grav. 1.180

What is the best concentration technique for concentration of cysts and eggs?

What are the thee cysts and eggs that are better concentrated by sedimentation than flotation.

The concentration technique destroys trophozoites, walls of eggs and cysts collapse, and organisms become distorted in appearance.

rapid deceleration may cause the floating organisms to sink to the bottom in this concentration technique.

This concentration technique cannot be sued for trematode and cestode ova.

This is the concentration technique of choice, because it is easier and causes less errors.

This sedimentation technique recovers protozoa, ova and larva, used on fresh or preserved specimens and iodine can be added for staining.

This sedimentation technique is less efficient than floatation for cysts and eggs, preserves most parasites, and is safer than formalin-ether technique.

This rapid sedimentation procedure removes lipids and colloidal material.

Ether is highly __________.

in macroscopic examination, hard or formed stool indicates what form of parasite.

loose, watery, liquid stool upon macroscopic examination usually indicates what form of parasite?

soft stool usually indicates what form of parasites?

what type of ova and larvae are found in any fecal consistency?

This test is preformed along side of macroscopic examination to detect occult blood.

Yellow and foul-smelling stool indicates what organism?

This organism may be found at the bottom of the container.

this ova may be found at the surface of the specimen.

if ______ is present, check for possible trophozoite forms.

These crystals are associated with amoebiasis and helminth infections.

When performing specimen examination scan the entire slide (wet mount) for ____ minutes on low power.

What is the direct mount used for motility.

What is the direct mount used for cyst (kills trophozoites)

What type of stain is used for trophozoites?

This procedure is preformed in conjuction with a concentration procedure, and is recommended for every fecal specimen.

This is a more convenient and permanent method compared to wet mounts.

What are the two preservatives that cannot be used for permanent slide stain?

This is a rapid, easy to use, highly stabe stain that can be used repeatedly, consistent.

What will be the appearance of cytoplasm, nucleus, and background material on the trichrome stain?

This is a complex, time consuming stain that requires experience.

what will the appearance fo cytoplasm, nucleus, and background using the Spencer-monroe stain?

what are five examples of pseudoparasites that can be falsely identified?

these stain purple and fuzzy and can be pseudoparasites in malarial smears.

after being centrifuged at 2300 for 1-2 min, what should be discarded in the flotation technique?

after adding zinc sulfate in the floatation technique, what should be done to the suspension?

What should be QC'd in the floatation technique?

in the sedimentation technique, 1-1.5 g of stool is added to 10% formalin and let stand for how long?

How long are sedimentation technique specimens centrifuged for?

What is used to resuspend in the sedimentation technique?

after the sedimentation technique is comple there will be four layers, how many should be discarded?

in the formalin-ethyl acetate procedure what should be checked for QC?

how is neutral pH maintained in the formalin preservative.

what are the four reagents and eqiupment for Trichrome (Gomori) stain?

this reagent in trichrome stain contains 1g K iodide in 100 ml DI H2O, and 1.5g iodine crystals which are filtered and sotred in a brown bottle.

How often should D'antoni's iodine be changed?

in the trichrome stain, fixed slides are placed in a bath of 70% alcohol for 2 minutes and _______ is added untill the mixture resembles strong tea.

known positive fecal specimens or feces containing buffy coat white cells (PVA preserved fecal specimens that have one or more intestinal protozoa are especially useful) is the QC for what?