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Introduction to Biotechnology
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What is recombinant DNA?
It is created when two pieces of DNA are joined together to form a new DNA molecule
What is a restriction enzyme?
It is used to cut DNA at a specific site
What do restriction enzymes generate?
Sticky or Blunt ends- for cloning
Fragmented DNA- can be used for profiling
What does a DNA ligase do?
Seals DNA into an opening created by the restrictions enzyme
What is the Southern Blot used for?
Technique to see specific polymorphic DNA fragments.
What is the Northern Blot
The transfer of RNA into nylon membrane (measure quality and quantity)
What is a vector?
DNA delivery vehicles
What are some types of vectors?
Plasmids
Phages
Cosmids
Eukaryotic Vector
Yeast artificial chromosome (YAC)
Bacterial Artificial chromosome (BAC)
What are Mammalian cell vectors?
Simian virus 40 (SV40)
Retrovirus
Adenovirus
What is cell transformation?
A process of cloning the recombinant DNA by putting into host cell such as
-E.coli
-Eukaryotic cells
cloning a Human Gene
How is Polymerase Chain Reaction used?
to amplify or increase DNA molecules invitro
What is needed for PCR?
Template (what you want to amplify)
Sequence-specific primers
Nucleotides
MgCl (enzyme cofactor)
Buffer
Taq
Polymerase
What are the steps in DNA amplification?
Denaturation- Separate DNA strands
Annealing- binding of primers to templet DNA
Extension- A new DNA strand created
Author
icimmy
ID
44711
Card Set
Introduction to Biotechnology
Description
Midterm 1 of Biotechnology
Updated
2010-10-25T03:33:28Z
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