pyrimidine nucleotide synthesis.txt

  1. What is the first pyrimidine formed?
  2. What is the first pyrimidine nucleotide formed?
  3. What activates and inhibits CPSII?
    • active = ATP, PRPP
    • inhibit = UTP
  4. What type of ribonucleosides are needed for DNA synthesis?
    diphosphates (ADP, GPD, CDP, UDP)
  5. What substrates can bind at the four substrate spots on Ribonucleotide reductase?
    • ATP, dATP, dTTP, dGTP
    • ATP = active
    • dATP = inhibition
  6. Why is ADA such a problem?
    • Converts Adenosine into ribo/deoxyribo through salvage
    • makes dATP which inhibits R.R. = lack of DNA production
  7. What is tetrahydrofolate utilized for (2 items)?
    • All purines
    • only dTMP for pyrimidines
  8. Why is folate deficiency dangerous?
    There is a link to cancer b/c of a lack of dTMP and more dUTP being incorporated into DNA
  9. What is the function of methotrexate?
    Inhibit DHFR
  10. What is the function of 5-fluorouracil?
    inhibit ThySyn by becoming f-dUMP and competing
  11. How does AZT Triphosphate work?
    Inhibits DNA replication by competing with dTTP
  12. What is the function of Trimethoprim?
    Inhibit bacterial DHFR
Card Set
pyrimidine nucleotide synthesis.txt
pyrimidine nucleotide synthesis