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220

  1. Acid-Fast Stain
    Add DI water to slide, then add tiny amount of culture.Dry and heat-fix
    Determine if Acid-fast or non acid-fast

    • Carbolfuchsin (primary stain)
    • Acid-alcohol (decolorizer)
    • Methylene blue (counterstain or secondary stain)
  2. Acid-Fast Stain
    Acid Fast Result: Red stained cells

    Non-Acid Fast Result: Blue stained cells
  3. Reagent: pH indicator phenol red
    End-Product: Acid products (Yellow color) / gas (Bubble)Positive Reaction: Yellow color or yellow color with large gas bubbleNegative Reaction: Red to orange color / no gas bubble
  4. Citrate

    Simmon’s citrate slant (bromthymol blue – pH indicator)
    End Product: Alkaline (Base) product
Author
ari_uzi
ID
41549
Card Set
220
Description
test
Updated

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