Micro Quiz 10/6/10

Mutation, Transformation, Conjugation, Transduction.

• Easier than in eukaryotes because of single chromosome
• Positive Selection
• Negative (indirect) selection

Rejects unmutated parental cells

• Replica Plating
• Negative (indirect selection)

• Method to identify potential carcinogens
• Most mutagens are carcinogenic
• Relies upon change in mutation rate

• Horizontal gene transfer: Between cells of the same generation. Can transfer DNA to its neighbor. It is usually the plasmid that is being shared.
• Vertical gene transfer: from one generation to the next
• Gene Expression: within a cell

Streptococis Pneumoniae. The one with a capsule (sugar coat) is virulent. A. immune system took care of Tye R nonvirulent. B. Type S virulent knew it would kill the mouse. C. boiled type S and inactivated it so it didn’t kill the mouse. D. Horizontal gene transfer. Contained Type R nonvirulent and boiled type S. Took up DNA from the dead ones and killed the mouse.