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Cell Fractionation
Taking Cells Apart
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Homogenization
Disruption of cells using a centrifuge
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Pellet
Larger, heavier structures that become packed at the bottom of a test tube during homogenization
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Supernatant
lighter cell parts, susupended in liquid above pellets during homogenization
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Eukaryotic Cells
membrane bound organelles
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Nuclear Envelope
seperates nucleus from cytoplasm, double membrane with pores
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Pore Complex
protein structure that lines pores
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Nuclear Lamina
protein filament net that maintains nucleus shape
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Chromatin
Stringy DNA, uncondensed
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Chromosomes
Condensed/coiled chromatin
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Chromatid
Intermediate step between chromatin and chromosomes
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Nucleolus
proteins/nucleic acids inside cell, ribosomal RNA synthesized/assembled
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Ribosomes
made of ribosomal RNA and proteins, carry out protein synthesis
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Endoplasmic Reticulum
Extensive membrane labyrinth
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Smooth Endoplasmic Reticulum
- surface lacks ribosomes, farthest from nucleus
- - Lipid synthesis (steroids)
- - Carbohydrate Metabolism
- - Detoxification
- - Calcium Ion Pumping - muscle contraction
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Rough Endoplasmic Reticulum
- synthesizes secretory proteins
- makes membrane
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Vesicles
sacs of membrane
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Golgi Apparatus
- modifies, packages, transports proteins
- - Cis Face - receiving end, near Endoplasmic Reticulum
- - Trans Face - shipping end
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Cisternae
stacks of flat sacs that make up the golgi apparatus
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Lysosomes
membrane bound sac of hydrolytic enzymes with pH of about 5
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Phagocytosis
cellular eating
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Autophagy
lysosome digests part of a cell
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Apoptosis
signaled cellular death by lysosome eruption
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Food Vacuole
sacs containing food
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Contractile Vacuole
freshwater protists - pump excess water out of cell
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Central Vacuole
plant cells, maintians rigidity
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Tonoplast
membrane enclosing central vacuole
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Mitochondria
cellular respiration, makes ATP
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Cristae
Folds in inner membrane of mitochondria
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Mitochondrial Matrix
inner part of mitochondria
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Animal
lysosomes, centrioles, flagella
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Plant
plasmodesmata, chloroplast, cell wall, central vacuole
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Chloroplast
makes energy, sugar through photosynthesis
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Stroma
fluid filled inner space of chloroplast
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Thylakoids
pancakes in chloroplast
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Grana
stacks of thylakoids in chloroplast
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Peroxisome
enzyme detosifies by transfering hydrogen to oxygen
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Cytoskeleton
microtubules, microfiliments, intermediate filaments
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Centrisome
form fibers to pull centrioles apart
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Centriole
pair of centrisome
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Centremere
hold chromatin together
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Micorvilli
fibers in intestine, absorb nutrients
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Pseudopodia
cellular movement by extensions/contractions
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Cystoplasmic Streaming
chloroplast movement to maximize light intake
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Middle Lamella
sticks cellular walls together between cells
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Extracellular Matrix
- outside of cell
- - Collagen
- - Proteoglycans
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Plasmodesmata
gaps in plant cells, passageways
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Tight Junctions
membranes fused together
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Desmosomes
anchoring junctions, rivets
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Gap Junction
cytoplasmic channels between adjacent cells
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Epethelial Tissues
outer lining
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