Gram Positive Bacilli 7.11

These are irregular, non-sporing gram positive rods that are coryneform or club shaped?

Corynebacterium are non-acid fast organisms that have what catalase result?

This bacteria is also known as Klebs-Loeffler bacillus

What are the three biotypes of C. diphtheriae?

What are the five species of Corynebacterium?

C. diphtheriae is spead by resiratory ___________, contact with _________ lesions or contaminated __________.

what type of toxins do C. diphtheriae produce?

Respiratory cytotoxins produced by C. diphtheriae cause what?

Cutaneous cytotoxins produced by C. diphtheriae cause what?

C. xerosis is a ____________ pathogen that causes endocarditis and septicemia.

C. pseudotuberculosis is also known as what?

This Corynebacterium causes pneumonia and granulomatous lymphadenitis?

This Corynebacterium causes Pneumonia, lung abcesses, and is associated with endocarditis.

These are GPRs that resemble and may be confused with C. diphtheriae, primarily opportunistic pathogens.

C. diphtheria is collected from what two sources?

What type of sawbs are used to collect C. diphtheria from nasopharyngeal and oropharyngeal cultures?

What three media are used to plate C. diphtheriae?

This media is used for primary isolation of C. diphtheriae.

what is the inhibitor in the Cystine-tellurite agar?

Growth of C. diphtheriae on the Cystine-tellurite agar will appear as what?

What biotypes of C. diphtheriae will appear as medium, white and opaque colonies on the BAP?

What biotype of C. diptheriae will appear as small gray and translucent colonies on the BAP?

This media supports growth for all species of Corynebacterium while inhibiting normal flora.

This media is a Cystine-sodium thiosulfate tellurite.

what will the appearance of Corynebacterium be on a modified tinsdale medium?

how is the Modified Tinsdale medium inoculated?

In this medium serum and egg stimulates production of metachromatic granules in Corynebacterium.

What stain is used for Crynebacterium?

what is the bacteria that has metachromatic granules?

C. diphtheriae displays what type of hemolysis on the BAP?

what causes blackening of the medium on the cystine tellurite agar?

Toxigenicity testing is used for isolated organisms to determine ________ state?

what Toxigenicty testing is used for In vitro organisms?

Toxigenicity testing is also known as what?

In this toxigenicity test paper stips with diptheria antitoxin are placed below the surface of agar.

What is the reult of 45^oC percipitation on the Elek immunodiffusion test?

What Toxigenicity test is used for In-vivo testing?

for in-vivo testing of C. diphtheriae, the first guinea pig is injected with what?

For in-vivo testing of C. diphtheriae, the second guinea pig is injected with what?

This Toxogenicity test is used to determine toxicity or neutralization of cytopathic effect.

These are Gram positive bacilli that are occasionally gram variable, asporogenous, short, coccobacillary to diphtheroidal and occasionally form chains.

What is the BEM result for Listeria?

what is the distinctive test for listeria?

This is a halotolerant bacteria that grows over a wide range of temperature on ordinary media.

What is the temperature range for Listeria?

Listeria can survive up to ____ NaCl at 4^oC for 8 weeks.

What is the glucose fermentation result of listeria?

What is the CAMP and H/H result of Listeria?

True or false
Listeria are regular, spore forming GPR.
True False

What are the species of Listeria?

What is the prime reservoir for Listeriosis?

This is a disease in a wide variety of fish, birds, and mammals, it is associated with abortion and encephalitis in sheep and cattle.

Listeriosis is caused by what bacteria?

This is an uncommon, but very significant human pathogen that causes bacteremia and meningitis in immunosuppresed, but only mild food poisoning in healthy persons.

True or false
Listeria monocytogenes can be transmitted congenitally.
True False

This bacteria can cause genital infection with habitual abortions.

This is when L. monocytogenes is transmitted by contact with infected animal tissues, usually in vets and livestock handlers. It is also associated with food borne transmission.

What is the catalase result for L. moncytogenes?

L. monocytogenes has what type of motility in the hanging drop method?

L. monocytogenes grows better at ________ than 35^oC.

This organism has a pinpoint or small, translucent, gray narrow zone of beta-hemolysis on the BAP. It also has an umbrelly type growth observed in semisolid motility media.

Listeria moncytogenes is often confused with what two bacteria? why?

This culture technique is used for L. monocytogenes specimens having heavily mixed flor (e.g. tissue)

For cold enrichment technique in L. moncytogenes how is the specimen incubated?

what technique of culturing L. moncytogenes requires continuous subculturing?

True or false
L. moncytogenes will stop growing when using the Cold Enrichment Technique.
True False

This GPR has a tendency to form long unbranched filaments, and may exhibit thickening and granular beading.

What is the TSI result that Erysipelothrix exhibits which is rare for a GPR?

what is the species in Erysipelothrix.

This is a swine pathogen that is seen in buthers, fisherman and vets.

What is the human disease caused by E. rhusiophathiae?

What is the pathogenicity infrequently seen in E. rhusiophathiae?

What are the three recommended specimens for processing E. rhusiophathiae?

This organism appears as pinpoint, circular, convex, transparent or larger rough colonies with matt surface and fimbriated edge that is slightly alpha or gamma hemolytic on the BAP.

Which GPR simulates miniature anthrax-type colonies on the BAP?

what is the motility result for E. rhusiophathiae?

what organism has a test tube brush pattern of growth in motility media?

Lactobacillus has a chain formation that is common and a tendency to form ________.

What is the organism that produces lactic acid from glucose as major metabolic end product?

what type of oxygen user is lactobacillus?

What is the GPR that is usually negative for catalase, motility, H2S and BEM?

This organism is prominent flora in the vagina, colon, mouth, and part of mixed flora.

This organism is seen in pleuropulmonary infections and dental caries.

What GPR grows well on BAP and CHOC and has a small and greenish alpha hemolytic zone on the BAP.

This organism is commercially used in the dairy industry.

What is this GPR?
Catalase: +
Motility: =
H2S: =
Esc: =
B hem: =

What is this GPR?
Catalse: +
Motility: +
H2S: =
Esc: +
B hem: +

what is this GPR?
Catalse: =
Motility: =
H2S: +
Esc: =
B hem: =

What is this GPR?
Catalase: =
motility: =
H2S: =
Esc: =
B hem: =

this ubiquitous, GPR, is endospore forming and has + motility.

which bacillus exists in air and dust, may be a common contaminant.

What are the three forms of B. anthracis?

what is the etiological agent of anthrax?

what Bacillus species may be used as a bioweapon because spores persist for long periods in contaminated materials.

What are the two types of food posioning caused by B. cereus?

This Bacillus causes two forms of food poisoning and traumatic eye infections.

This bacteria is used as sterility control for steam autoclave procedures and food canning.

B. stearothermophilus is a thermophilic bacteria that grows at what temp?

true or false
B. stearothermophilus spores occur in soil in all climactic zones.
True False

These are large to med, flat, irregular, non-hemolytic, light grey GPR, that may have a ground glass appearance on older cultures.

when viewed under dissecting microscope B. anthracis has serrated margis with what classical appearance?

B. anthracis colonies show ______ when manipulated on BAP?

this bacillus closely resembles Pseudomonas on the BAP?

any isolate of Bacillus morphologically suggestive of B. anthracis should immediately be submitted to who?

This Bacillus appears as long chains resembling a jointed bamboo rod and has extensive sporulation with a gram stain.

These are standardized spore suspension culutre mediums with a pH indicator.

what is the pH indicator on the sealed glass ampule (BBL "killit")?

this is filter paper strips impregnated with spores, dehydrated culture media, and pH indicator.

turbidity indicates what result in BBL "killit"?

Growth + on BBL "killit" indicates what?