1. What is Fuzeon? What does it have to do with HIV?
    • Fuzeon is an entry inhibitor.
    • Prevents the fusion of HIV--> used for drug resistant HIV infection
  2. Mechanism of Fuzeon
    • pg120 binds to CD4-->
    • conformational change-->
    • hydrophobic region of gp41 exposed-->
    • virus membrane fuses host cell membrane
  3. What are the characterics of rash?
    • Abundant on face, forearm, lower limbs
    • Sparce on torso

    Umbilicated: indent on the postule

    *postules are caused by immune response*
  4. Why was it possible to eradicate smallpox?
    • It's a DNA virus
    • (humans host ONLY)
  5. How is Molluscum contagiosum manifestated?
    • Image Upload 2
    • Umbilicated nodules: waxy, pearly w plug when squeezed
    • Aggregated in an area-- cluster of 5-20
    • Cytoplasmic inclusion: cytoplasm of infected cell full of inclusions
  6. What is Cytoplasmic inclusions?
    • Nucleus is pushed to the side
    • Cell becomes LARGER than normal

    • Inclusion body: viral accumulation in cellular compartment
    • Ex:
    • CMV
    • Herpes
  7. How is Molluscum contagiosum transmitted?
    • Common in the tropics: Can be easily spread from towel to towel
    • Sexual contact: nodules in pubic area
  8. List the different kinds of vaccines
    • Inactivated: contain inactivated viruses
    • Attenuated/live: contain altered viruses so they can't cause disease
    • Recombinant protein
    • Live vaccine
  9. What is the structure and nature of transmitting HPV?
    • Naked, icosahedral DNA virus
    • Transmitted from formites, direct contact, and sexual contact
  10. Where does the HPVirus replicate in the skin?
    • HPV infects epithelial cells of skin or
    • mucous membranes
  11. Which is NOT true regarding adsorption?

    a) Requires ATP
    b) Virus attaches to host membrane
    c) Nonenveloped viruses attach via exposed capsid regions
    d) Process is random and reversible
    e) All
    a) Requires ATP
  12. Viruses are classified according to all of the following EXCEPT:

    a) Type of replication
    b) Structure of virion
    c) Host required for survival
    d) Type of nucleic acid
    e) Structure of virus
    c) Host required for survival
  13. Which of the following is NOT true regarding caspids?

    a) Able to self-assemble into virions
    b)Helical structure has fixed number of subunits and is spherical in shape
    c) composed of repeating subunits
    d) Only A and B
    e) All
    d) Only A and B
  14. A particular virus' genetic material first needs to be copied
    into positive sense SS-RNA (Single Strand RNA). What type of virus is this?
    • a) SS Positive-Sense RNA
    • b) SS Negative-Sense RNA
    • c) DS RNA
    • d) DS DNA
  15. A particular virus' genetic material first needs to be copied
    into positive sense SS-RNA (Single Strand RNA). What type of virus is this?
    c) DS RNA
  16. Adenoviidae, Poxviridae, and Herpesviridae are
    Double-stranded DNA
  17. What type of immunity is most responsible for fighting viral infections
    Cell-Mediated Immunity
    All viruses have a protein caspid AND an envelope
    • FALSE
    • some viruses do no possess envelope
    AIDS is caused by HIV
    Viral attachment protein and protein receptor are the same
    • FALSE
    • VAP is the viral protein which attaches to the host cell from the virus
    • Cellular protein is the receptor on host cell surface to which VAP attaches
    Rhinovirus is naked DNA virus and has an icosahedral shape
    • FALSE
    • Rhinovirus is an (+)ssRNA, naked w icosahedral shape

    Adenovirus is naked DNA virus w icosahedral shape
  22. Transmission of Adenovirus
    • Aerosol,
    • close contact,
    • fecal-->oral,
    • contaminated swimming pool
  23. What does Adenovirus infect?
    • Mucoepithelial cells in respiratory tract
    • GI tract
    • conjuctiva or cornea

    *causes cell damage directly since VIRUS PERSIST IN LYMPHOID TISSUE
  24. Common infections caused by Adenovirus
    • 1. Upper Respiratory Tract:
    • a) Acute febrile pharyngitis- strep throat
    • b) Pneumonia-
    • Permanent lung damage
    • Measles predisposes children to more severe Adenovirus
    • 2. Conjuctivitis: pink eye--> HIGHLY infectious
    • 3. Tonsillitis: inflammation of the tonsils
    • a) unresponsive to antibiotic therapy
    • b) blood chemistry is the same fro viral and bacterial tonsillitis
    • 4. Hemorrhagic cystitis:
    • a) bladder infection
    • b) bleeding of the bladder
    • 5. Gastroenteritis: GI tract
    • a) watery non bloody diarrhea
  25. Adenovirus portal of entry
    • Respiratory tract: olfactory route
    • Alimentary canal
    • Skin
  26. Adenovirus site of shedding
    • Respiratory/coughing
    • Skin lessions: Herpes, HPV- wart
    • Fecal to oral: HepA, Polio
    • Secretion: blood/semen
    • Nosocomial: spread infection in the hospital
  27. Whats the difference btwn shedding and spreading?
    Shedding: outside the body

    Spreading: spread through local tissue w/i body
  28. Which of the following viruses can cross the placenta?
    a) Rubella
    b) HIV
    c) CMV
    d) B and C only
    e) All
    e) All
  29. What are neurotropic viruses?
    • Viruses that move along the neurons
    • Ex:
    • Polio: damage to motor neuron--> paralysis
    • Herpes: to brain via olfactory nerve (nose)
  30. Viral pathways to brain
    • 1) Neural: Polio, Yellow fever, Rabbies, Herpes 1&2
    • 2) Olfactory: Herpes and Coronavirus
    • 3) Hematogenous: Polio, Mumps, CMV, and Herpes
  31. Localized infections. Give examples.
    do NOT spread in the body, shed the SAME place they entered

    Ex. Rhinovirus, Influenza, Adenovirus
  32. Generalized infections. Give examples.
    Spread in other tissues, shed through DIFFERENT entry

    Ex: Measles, Rubella, Varicella
    Viremia is an active virus
    • FALSE
    • it is passive, no nucleus in RBCs
  34. What is Syncytia?
    • accumulaiton of viral antigens in host membrane
    • causes fusion of cells resulting in LARGE multinucleated cell
    • enveloped virus ONLY

    Ex: Herpes on cervix and Measles
  35. Viruses and Cancer
    • HIV
    • Epstein barr
    • HepB
    • HepC: liver cancer
    • HTLV
    • HPV
  36. Which of the following uses the Respiratory Tract as a portal of entry but causes generalized infection?
    • Mumps
    • Measles
    • Rubella
  37. Viruses caused by breaks in the skin
    • Minor trauma
    • Herpes Simplex
    • HepB
    • insect bites
    • Injections
    • HepB
    • HIV, HTLV
    • CMV
    • Ebola
    • Animal bites
    • Rabbies
    • HerB
    • Genital tract
    • Herpes simplex
    • HIV, HTLV
    • HepB nnd C
    • Conjuctiva
    • Adenoviruses
    • Enterovirus 70
  38. What is the difference between naked and enveloped viruses?
    Naked: non-enveloped viruses are more resistant to drugs.

    Enveloped viruses are more fragile and easily deactivated. Their envelope is composed of a lipid bilayer from host's membrane.
  39. Give examples of naked viruses
    • Retroviruses,
    • Rotaviruses,
    • rhinoviruses,
    • poliovirused,
    • Erboviruses,
    • Norwalk virus,
    • HPV,
    • Adenovirus
  40. Give examples of enveloped viruses
    • Smallpox- viriolla virus,
    • herpes simplex virus,
    • varicella- zoster virus,
    • Cytomegalovirus
    • Epstein- barr virus,
    • Measles, mumps, rubella virus,
    • Respiratory syncytial virus,
    • Influenza virus
    • Hepatitis, rabies
  41. RNA viruses...
    High rate of mutation, drug resistant.

    Heterogeneous: cross btwn human and animals.
  42. RNA viruses- positive vs. negative
    Positive: can be translated into a protein.

    Negative: template that has to be transcribed into a + RNA b4 translation into protein.
  43. What is an RNA-dependent-DNA-polymerase?
    Reverse transcriptase (virus ONLY--> RNA» DNA)
  44. DNA viruses...
    Human only, evolve with host
  45. DNA viruses examples
    • Adenovirus
    • HPV
    • Herpes simplex
    • Varicella- zoster
    • Cytomegalovirus
    • Epstein- Barr
    • Hepatitis B
    • Smallpox, cow, and monkey viruses
    • Vaccinia virus
  46. True/false:
    Envelope of enveloped viruses ONLY contain proteins of host origin OR viral origin
    FALSE-- proteins of both the host AND virus
  47. What structure is important in recognition? Example?
    Cell-cell interactions and host-cell recognition when glycoproteins are present

    • Influenza:
    • Neuraminidase- found on surface of Inf. Cleaves residue from Sialic acid. Allows detachment and spread.

    • Hemagglutinin- viral attachment protein. Binds to Sialic acid.
    • Sialic acid: receptor found in mucoepithelial cells

    • HIV:
    • gp120- recognizes CD4
    • Ngp41- functions in additio
  48. Which of the following is a complex virus. What is a complex virus?
    Complex virus: caspid composed of protein bilayer

    • Examples:
    • Reovirus.
    • Viruses belonging to the baculovirus family.
    • Herpes, pox viruses
  49. Reassortment
    • Segmented genomes, which can be "shuffled" during superinfection.
    • Ex:
    • influenza virus: 8 different RNA molecules, only infect w 2--» new strains
  50. Define caspid
    • Protein coat that packages and stores nucleic acid
    • Protection of viral genome from environment
    • Maybe involved in host-cell recognition and dumping mechanism
  51. Helical nucleocaspid
    Caspid where proteins interact w nucleic acid

    • Ex:
    • Rabies
    • Tobacco mosaic
    • Mumps
    • Measles
    • Influenza
  52. Octahedral caspid
    • Icosohedral geometry: from protective shell around caspid
    • Common among viruses that infects humans- DNA virus- minimal free energy structure

    • Ex:
    • Adenovirus
    • Rhinovirus
  53. What's the importance of glycoproteins on surface of virus?
    • Enveloped- viral attachment
    • Naked- surface peptide

    Establish tropism
  54. Tropism
    Virus specific for its host/cell type
  55. What is and in which virus is Hemagglutinin?
    • It's a glycoprotein- viral attachment protein.
    • Binds to Sialic acid.
    • Receptor found in mucoepithelial cells.
  56. What is, where is it found, what does Neuronaminidase do?
    • It is a glycoprotein.
    • Found on surface of influenza virus.
    • Cleaves galactose residue from Sialic acid.
    • Allows for detachment and spread
  57. What's the role of H1N1 glycoproteins?
    Neutralize antibodies
  58. Intramuscular vs. oral polio vaccine
    • IM is better b/c there's no risk of vaccination turning virulent.
    • IM inactivated vaccine no reversion
  59. Antigenic drift

    "subtle" changes over time
  60. Antigenic shift
    SUDDEN changes in genetic composition

    Ex: reassortment of influenza
  61. Pandemic
    Under antigenic shift--» Mutational adaptation and reassortment

    50% of genome can mutate w/o affecting its replication
  62. What are the 3 types of penetration?
    • 1. Fusion: virus fused with host cell membrane
    • Ex: enveloped viruses only

    • 2. Endocytosis: internalization of coated pits at cell membrane
    • Uptake of virus inside the cell

    3. Translocation: receptors carries virus across the membrane
  63. Which of the 3 types of penetration is the most common?
  64. Which of the 3 types of penetration is rare?
  65. What are the stages in replication?
    • 1. Attachment
    • 2. Penetration
    • 3. Uncoating
    • 4. Genomic replication
    • 5. Gene expression
    • 6. Assembly
    • 7. Release
  66. What are the molecules in charge of preventing attachment?
    Neutralizing bodies
  67. VAP
    • virus attachment protein- binds to receptor on cell
    • Determines tropism
  68. Interferon
    1st line of defense- infected cell-> signal to other cells-> interferon release->attach to receptors of "healthy" cells
  69. What does interferon do when activated? Give example
    • Induction of transcription of interferon gene--» induction of immune system response (ache & pain)
    • Shut down protein synthesis in cell

    Ex: used in treatment of Hepatitis C
  70. What is Crixivan and what does it do?
    It is a protease inhibitor that prevents maturation

    Its protease activity cleave protein substrate (viral or cellular)
  71. What is AZT (NRTI) and what does it do?
    NRTI- nucleoside reverse transcriptase inhibitor: RT inhibitor found on HIV and HepB
  72. What does AZT do?
    • It targets replication of genomic material
    • leads to premature termination
  73. what is Ribavirin used for?
    To prevent replication of viruses by introducing mutations

    It is a polymerase inhibitor-- RNA virus
  74. What's the role of vaccines?
    To stimulate the development of circulating antibody against the caspid/envelope proteins of virus
  75. Variola
    Major and minor causes of chicken pox
  76. Vaccinia
    • Comes from cowpox
    • Propagated in the lab
  77. Incubation periods
    • rhinovirus: 1-3 days
    • Chickenpox: 13-17 days
    • HepA: 15-40 days
    • HepB: 40-150 days
    • herpes simplex: 5-8 days
    • HIV (AIDS): 1-10 years
Card Set
Exam 1