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magnification
ratio of an object's image size to its real size
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light microscope (LM)
- -most common microscope in the lab
- -visible light is passed through the specimen and then through the glass lenses
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resolution
measure of the clarity of the image
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electron microscope
- magnification 100,000x
- electron beam in a vaccum focused by magnets
- electron beam has much shorter wavelength than visible light
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Scanning electron microscope (SEM)
- provides for detailed studies of the surface of a specimen
- thing getting studied often covered in gold
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transmission electron microscope (TEM)
study of intermal ultrastructure of cells
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cell fractionation
- uses centrifuge
- takes cells apart and separates the major organelles and other subcellular structures from one another
- forms pellets on the bottom
- most dense pellet out first
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plasma membrane
selective barrier that bounds and protects all cells
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Where does cellular respiration occur?
Mitochondria
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Where is ATP produced?
mitochondria
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What organelle is responsible for photosynthesis?
chloroplasts
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chloroplast
- plant cell
- photosynthetic organelle
- converts energy of sunlight to chemical energy stored in sugar molecules
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plasmodesmata
- plant cells
- channels through cell walls that connect the cytoplasms of adjacent cells
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central vacuole
- plant cells
- prominent organelle in older plant cells
- functions include storage, breakdown of waste products, hydrolysis of macromolecules
- enlargement of vacuole is a major mechanism of plant growth
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lysosome
- membranous sac that animal uses to digest molecules
- acidic environment
- recycles cell's organic molecules
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mitochondrion
- plant and animal cells
- organelle where cellular respiration occurs and ATP is generated
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golgi apparatus
- plant and animal cells
- products of ER are modified and stored and then sent to other destinations
- consist of cisternae-flattened membranous sacs resembling stacks of pita bread
- two poles of stack are called cis face and trans face
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flagellum
- animal cell
- locomotion organelle present in some animal cells
- composed of a cluster of microtubules within an extension of the plasma membrane
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gap junction
a type of intercellular junction in animals that allows the passage of materials between cells
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contrast
accentuates differences in parts of the sample
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Ribosome
- complexes made of ribosomal RNA and protein
- carry out protein synthesis
- free or bound
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What are the components of the endomembrane system?
nuclear envelope, endoplasmic reticulum, Golgi apparatus, lysosomes, vacuoles, and the plasma membrane
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endoplasmic reticulum (ER)
- network of membranous tubules and sacs called cisternae
- separates internal compartment of the ER from the cytosol
- smooth and rough
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smooth ER
- metabolic processes: synthesis of lipids, metabolism of carbs, detox of drugs and poisons
- stores calcium ions
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rough ER
- makes secretory proteins
- grows in place by adding membrane proteins and phospholipids to its own membrane
- have ribosomes attached
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thylakoid
flattened, interconnected sacs in the chloroplast
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granum
individual stack of thylakoids
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stroma
- fluid outside the thylakoids
- contains chloroplast DNA and ribosomes as well as many enzymes
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cytoskeleton
- network of fibers extending throughout the cytoplasm
- made up of microtubules, microfilaments, and intermediate filaments
- support, mobility, and regulation
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microtubules
- thickest of the three components of the cytoskeleton
- wall of this tube is filled with tubulin
- shape and support the cell
- serve as tracks along which organelles equipped with motor proteins can move
- can separate chromosomes during cell division
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microfilaments
- built from molecules of actin
- present in all eukaryotic cells
- cell motility
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intermediate filaments
- more permanent fixtures of cells than microfilaments and microtubules
- functions as the framework of the entire cytoskeleton
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cell wall
- extracellular structure of plant cell
- protects plant cell, maintains its shape, and prevents excessive uptake of water
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amphipathic
- hydrophilic region and hydrophobic region
- most proteins within membranes are amphipathic
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fluid mosaic model
the membrane is a fluid structure with a "mosaic" of various proteins embedded in or attached to a bilayer of phospholipids
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desmosome
fasten cells together into strong sheets
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tight junction
- plasma membrane of neighboring cells are very tightly pressed against each other, bound together by specific proteins
- prevent leakage of extracellular fluid across a layer of epithelial cells
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What are the most abundant lipids in most membranes?
Phospholipids
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What are the two major types of membrane proteins?
Integral and peripheral
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integral proteins
penetrate hydrophobic core of lipid bilayer
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peripheral proteins
appendages lossely bound to the surface of the membrane, often exposed to parts of integral proteins
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channel proteins
hydrophobic channel that certain molecules or atomic ions use as a tunnel through the membrane
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diffusion
- movement of molecules of any substance so that they spread out evenly into the available space
- more concentration to less concentration
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passive transport
- diffusion of a substance across a biological membrane
- cell doesnt have to expend energy ot make it happen
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osmosis
diffusion of water across a selectively permeable membrane
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tonicity
the ability of a solution to cause a cell to gain or lose water
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isotonic
- same environment for the cell and its environment
- no net movement of water acorss the plasma membrane
- water flows at the same rate in both directions
- volume of cell is stable
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hypertonic
- water will lose water to environment
- shrivel and die
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hypotonic
- water will enter cell faster than it leaves
- cell will swell and burst
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turgid
swollen or distended as in plant cells (A walled cell becomes turgid if it has a greater solute concentration than its surroundings, resulting in entry of water)
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flaccid
- limp
- plant cell becomes flaccid when the cells and surroundings are isotonic
- no tendency for water to enter
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facilitated diffusion
the spontaneous passage of molecules or ions across a biological membrane with the assistance of specific transmembrane transport proteins
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What are the two types of transport proteins?
- channel proteins
- carrier proteins
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channel proteins
provide corridors that allow a specific molecule or ion to cross the membrane
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