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When investigating the growth of leaf yeast using agar plates and controls, how many steps are there?
- 13 steps
- 1. Collect leaves
- 2. Wash, disinfect and sterilise corker borer
- 3. 2 Agar plates A and B
- 4. Cut discs from leaf with cork borer
- 5. Plate A upside down
- 6. Flame forceps and allow to cool
- 7. Raise base of petri dish
- 8. Smear petroleum jelly onto inside of lid
- 9. Attach leaf disc to each blob of jelly. Relate forceps
- 10. Seal plates with parafilm. Label+date
- 11. Store petri dishes in incubator @20*, 24hrs
- 12. Turn petri dishes upside down, incubate @20* for 3 days
- 13. Expected results: pink colonies grew
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When investigating the growth of leaf yeast using agar plates and controls, what is the expected result?
Pink colonies grew
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When investigating the growth of leaf yeast using agar plates and controls, what safety precautions are taken at beginning?
- Tie back hair and wash hands
- Wash and disinfect the bench
- Flame the cork borer to sterilise
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When investigating the growth of leaf yeast using agar plates and controls, what are the two agar plates called?
Labelled A and B
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When investigating the growth of leaf yeast using agar plates and controls, what is used to cut discs out of leaf?
Cork borer
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When investigating the growth of leaf yeast using agar plates and controls, how is the forceps sterilised?
Are flamed with Bunsen burner
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When investigating the growth of leaf yeast using agar plates and controls, how is the petri dish handled to add the petroleum jelly and leaf discs?
- The base of the Petri dish (petri dish is upside down) is carefully raised just enough to work with.
- Using forceps smear dots of petroleum jelly onto inside of the lid (Vaseline sticks leaf to lid).
- Use forceps to attach a leaf disc to each blob of jelly.
- Reflame forceps
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When investigating the growth of leaf yeast using agar plates and controls, what happens before the petri dishes are placed in incubator?
- Seal plates with parafilm
- Label and date both plates
- Incubator is at 20*C
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When investigating the growth of leaf yeast using agar plates and controls, firstly how are the petri dishes stored?
(First round) Petri dishes right way up in an incubator at 20*C for 24 hours.
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When investigating the growth of leaf yeast using agar plates and controls,secondly how are the petri dishes stored?
Turn Petri dishes upside down and incubate at 20*C for 3 days to prevent condensation and yeast can fall down
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When investigating the growth of leaf yeast using agar plates and controls, why are the petri dishes turned upside down after 24 hrs for another 3 days?
To prevent condensation, yeast can fall down.
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When investigating the growth of leaf yeast using agar plates and controls, what is done on completion?
The agar plates were sterilised by placing them in a disinfectant for 24 hrs and then placed in bin.
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What is a nutrient medium?
Nutrient medium: (agar) is a material on which yeast can grow as it provides a supply of food necessary for the yeast.
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What are some (other than yeast) fungi?
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When investigating the growth of leaf yeast using agar plates and controls, what equipment is used?
- Ash leaf
- Cork borer
- Disinfectant (firstly, for bench and then agar plates at end)
- Agar plates
- Bunsen burner (to sterilise cork borer and forceps)
- Forceps
- Petroleum jelly
- Parafilm (to seal the plates)
- Incubator (store petri pishes at 20*)
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