MICROPARA LAB

one of the basic tools used in the conduct of both basic and advance biological research.

The microscope is one of the basic tools used in the conduct of both?

The common light microscope used in the laboratory is called a?

Why is it called a compound microscope?

The lens closest to the eye is called the

the lens closest to the object is called the

Most microscopes have on their base an apparatus called a ____, which condenses light rays to a strong beam.

located on the condenser controls the amount of light coming through it.

Both coarse and fine adjustments are found on the?

What adjustments are found on the light microscope?

How to compute the total magnification possible with the microscope?

A compound light microscope often contains how many lenses and what are they with number

Ocular lens magnifies _ times than the objective lens

With an ocular lens that magnifies 10 times, the total magnifications possible will be?

Most microscopes are ____. This term means that the microscope remains in focus when one switches from one objective to the next objective.

The ability to see clearly two items as separate objects under the microscope is called the?

The resolution is determined in part by the?

Visible light has a wavelength of about?

ultraviolet light has a wavelength of about?

The resolution of a microscope increases as the?

allows one to detect objects not seen with visible light.

The resolving power of a lens refers to the?

based on the wavelength of the light used and the numerical aperture of the lens.

Refers to the size of the smallest object that can be seen with that lens

refers to the widest cone of light that can enter the lens; is engraved on the side of the objective lens.

If the user is to see objects clearly, ____must enter the objective lens.

is exceedingly narrow, and most light misses it. Therefore, the object is seen poorly and without resolution.

To increase the resolution with the oil‐immersion lens, a drop of___ is placed between the lens and the glass slide.

To increase the resolution with the oil‐immersion lens, a drop of immersion oil is placed between?

has the same light‐bending ability (index of refraction) as the glass slide, so it keeps light in a straight line as it passes through the glass slide to the oil and on to the glass of the objective

With the increased amount of light entering the objective, the resolution of the object?

To properly calibrate your eyepiece with a stage micrometer what lens should you use?

Calibrate using what micrometers?

During microscopic observation of cells, for bigger specimen, the ___ may be used and for smaller ones, the ___may be used.

What should be calibrated first?

The survival and growth of microorganisms depend on?

The general composition of a medium is as follows:

According to the consistency three types of media are used:

Liquid media, such as?

Liquid media, such as nutrient broth, tryptic soy broth or glucose broth can be used in studies of?

Tubes and flasks with liquid cultures can be incubated with what incubation?

Solid media can be poured into either?

What media can be poured into either a test tube or Petri dish.

If the medium in the test tube is allowed to harden in a slanted position, the tube is designated an?

if the tube is allowed to harden in an upright position, the tube is designated an?

if the agar is poured into a Petri dish, the plate is designated an?

media that are composed of known quantity and quality of pure chemicals

Medias that are categorized by their composition

In routine bacteriology laboratory exercises, these are employed. These are composed of complex materials rich in vitamins and nutrients, the chemical composition of which is poorly defined.

3 of the most commonly used components in complex or nonsynthetic media are

Medias that are categorized by their function

Such as Tryptic Soy Agar, supports the growth of most bacteria cultured in the laboratory. They do not contain any special additives.

media enhance the growth of certain organisms while inhibiting the growth of others due to the inclusion of particular substrate.

media allow identification of microorganisms usually through the (visible) physiological reactions unique to those bacteria.

media allow metabolically fastidious microorganisms to grow because of the addition of specific growth factors.

one obtained with the use of selected media and incubation conditions to isolate the desired microorganisms from natural samples.

Culture broth can be dispensed with the?

employs many techniques and skills when handling microorganisms.

Isolation and investigation of microorganisms
are formidable tasks confronting both novice and experienced microbiologists. The skills employed to achieve these tasks are called

are formidable tasks confronting both novice and experienced microbiologists.

reduced the potential spread of bacteria used in laboratory to you or other people.

is necessary to prevent contamination of pure cultures (a culture containing a single species) which are used routinely for analysis.

a culture containing a single species

is a powerful technique that microbiologists employ for a number of purposes, including 1) evaluating the purity of a bacterial culture, 2) examining the diversity of species in a sample, 3) separating a species from a mixed culture so that a pure culture can be prepared, and 4) studying the colony characteristics of a species.

is carried out to obtain isolated colonies.

is a bacterial growth on solid medium that originated from a single cell.