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BioTech Weeks 9,10,11

  1. Using liposomes to deliver DNA into a cell is best described as:



    C) Transfection
  2. What is Lipofection?
    Image Upload 2
  3. What is Viral Transduction?
    Image Upload 4
  4. What is Calcium Phosphate Precipitation?
    Image Upload 6
  5. How do you transfer DNA into mammalian cells by physical means?
    Image Upload 8
  6. What are some variables that influence transfection efficiency?
    • - Cell confluency
    • - Cell type
    • - DNA:transfection reagent
    • - DNA mass
    • - DNA purity
    • - Cell age
    • - Cell cycle status
  7. When maintaining suspension cells, is it necessary to use trying when passing? Why or why not?
    No

    Trypsin is a protease enzyme that cleaves or breaks down proteins. This must be used in adherent cell sub culturing protocols to disrupts the cell adhesion that compose cell-cell and cell-surface protein interactions. Suspension cells on the other hand do not make cell-cell or cell-surface protein interactions, so treatment with trypsin isn't necessary.
  8. How do cells detect and respond to an environment?(3 steps)
    • 1. Reception of the signaling model
    • 2. Transduction relays signals from the receptors to target molecules
    • 3. Response change in transcription or cytoplasmic activity
  9. What is a receptor?
    bind to signaling molecules to initiate a physiological response
  10. GPCRs
    G-protein coupled receptors
  11. Three types of ways for the ligand-substance interaction?
    Step 1- reception of the signaling model
    • GPCRs (G-protein coupled proteins)
    • Ion channeling
    • RTKs receptor tyro-sine kinase
  12. ligand
    another name for a signaling molecule
Author
saucyocelot
ID
361184
Card Set
BioTech Weeks 9,10,11
Description
Cell culture and protein purification, cell signaling
Updated

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