BioTech Weeks 9,10,11

Using liposomes to deliver DNA into a cell is best described as:

C) Transfection
What is Lipofection?
What is Viral Transduction?
What is Calcium Phosphate Precipitation?
How do you transfer DNA into mammalian cells by physical means?
What are some variables that influence transfection efficiency?
- - Cell confluency
- - Cell type
- - DNA:transfection reagent
- - DNA mass
- - DNA purity
- - Cell age
- - Cell cycle status
When maintaining suspension cells, is it necessary to use trying when passing? Why or why not?

No

Trypsin is a protease enzyme that cleaves or breaks down proteins. This must be used in adherent cell sub culturing protocols to disrupts the cell adhesion that compose cell-cell and cell-surface protein interactions. Suspension cells on the other hand do not make cell-cell or cell-surface protein interactions, so treatment with trypsin isn't necessary.
How do cells detect and respond to an environment?(3 steps)
- 1. Reception of the signaling model
- 2. Transduction relays signals from the receptors to target molecules
- 3. Response change in transcription or cytoplasmic activity
What is a receptor?

bind to signaling molecules to initiate a physiological response
GPCRs

G-protein coupled receptors
Three types of ways for the ligand-substance interaction?
Step 1- reception of the signaling model
- GPCRs (G-protein coupled proteins)
- Ion channeling
- RTKs receptor tyro-sine kinase
ligand

another name for a signaling molecule

Author

saucyocelot

361184

Card Set

BioTech Weeks 9,10,11

Description

Cell culture and protein purification, cell signaling

Updated

2023-09-26T02:20:40Z